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Protocols
  • Determination of Ncleotide Composition of RNA (Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
    Determine the concentration of each base in RNA sample by UV spectrophotometer
    http://homepages.gac.edu/~cellab/chpts/chpt14/ex14...
    Added: Tue May 14 2002, Hits: 198, Reviews: 0  Write review   Cached
  • How to prepare Molecular Biology grade glycogen (Contributed by Roberto Salvi)
    Glycogen can conveniently substitute for tRNA as a carrier for nucleic acid precipitation. Although Molecular Biology grade glycogen can be purchased from a number of vendors, the main disadvantage is that it is very expensive (e.g., About 100 dollars/20-40 mg). Here we present a simple and inexpensive protocol to prepare a large amount of glycogen which is suitable for any kind of Molecular Biology application (we routinely use it for RNA work).
    Added: Tue May 14 2002, Reviews: 0  Write review  
  • Orcinol Determination of RNA (Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
    http://homepages.gac.edu/~cellab/chpts/chpt14/ex14...
    Added: Tue May 14 2002, Hits: 207, Reviews: 0  Write review   Cached
  • Recovery of RNA fragments from Polyacrylamide gels (Beverly Faulkner-Jones)
    The best method is the crush and soak method. It can be sued to isolate single-stranded RNA from denaturing gels. The RNA is of high purity but the method is long and inefficient if long transcripts are to be recovered (cf. less than 30% of DNA greater than 3 kb is recovered).
    http://grimwade.biochem.unimelb.edu.au/bfjones/gen...
    Added: Tue May 14 2002, Hits: 134, Reviews: 0  Write review   Cached
  • RNA Conversions (Ambion)
    When isolating RNA, it helps to have a rough idea of how much total or poly(A) RNA can be recovered from a given amount of tissue or cells.These numbers provide a guideline against which experimental procedures and results can be checked.
    http://www.ambion.com/techlib/tips/rna-conversions...
    Added: Tue May 14 2002, Hits: 175, Reviews: 0  Write review   Cached
  • Spectrophotometric Analysis of RNA (Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
    http://homepages.gac.edu/~cellab/chpts/chpt14/ex14...
    Added: Tue May 14 2002, Hits: 306, Reviews: 0  Write review   Cached
  • Sucrose Density fractionation of RNA (Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
    http://homepages.gac.edu/~cellab/chpts/chpt14/ex14...
    Added: Tue May 14 2002, Hits: 177, Reviews: 0  Write review   Cached