Top : Molecular Biology : Protein : Protein Electrophoresis : SDS-PAGE

Summary: SDS-PAGE stands for Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and is a method used to separate proteins according to their size. Since different proteins with similar molecular weights may migrate differently due to their differences in secondary, tertiary or quaternary structure, SDS, an anionic detergent, is used in SDS-PAGE to reduce proteins to their primary (linearized) structure and coat them with uniform negative charges. Procedures for SDS-PAGE generally involve 1) making a gel and assembling the gel apparatus, 2) mixing protein samples with sample buffer containing SDS and heat the mixture at high temperature, 3) loading samples and running the electrophoresis, 4) fixing and staining the separated proteins.
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Protocols
  • Introduction to SDS-PAGE (David R. Caprette, Rice University )
    Very nice and basic guide to SDS-PAGE gel electrophoresis technique.
    http://www.ruf.rice.edu/%7Ebioslabs/studies/sds-pa...
    Added: Sun Sep 29 2002, Hits: 20864, Reviews: 0  Write review   Cached
  • SDS-Page Gel Electrophoresis (School of Chem. and Biochem, Georgia Institutue of Technology)
    Polyacrylamide gel electrophoresis (PAGE) is probably the most common analytical technique used to separate and characterize proteins. This protocol provides detailed procedures and recipes with illustrations.
    http://ww2.chemistry.gatech.edu/~lw26/bCourse_Info...
    Added: Sat Aug 04 2012, Hits: 4507, Reviews: 0  Write review  
  • Denaturing Discontinuous Polyacrylamide Gel Electrophoresis (SDS-PAGE) (Goldberg Lab)
    Very nice and detailed protocol
    http://wheat.pw.usda.gov/~lazo/methods/goldberg/sd...
    Added: Tue May 14 2002, Hits: 4672, Reviews: 0  Write review   Cached
  • PAGE-SDS Laemmli Protocol (Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
    http://wolfson.huji.ac.il/purification/Protocols/P...
    Added: Sat Feb 21 2009, Hits: 8880, Reviews: 0  Write review   Cached
  • Protocol for SDS-PAGE Analysis (Protocol Online)
    This protocol described the validated protocol in our lab for SDS-PAGE analysis of proteins.
    Added: Thu Feb 28 2013, Reviews: 0  Write review  
  • SDS Gel Electrophoresis (Proteinchemist.com)
    http://www.proteinchemist.com/tutorial/sdspage.htm...
    Added: Sat Jan 31 2009, Hits: 10028, Reviews: 0  Write review   Cached
  • SDS-PAGE Protocol (Immuno-Physiology Laboratory, FLU)
    http://www.fiu.edu/~animals/protocols/sds_page.htm...
    Added: Sat Jan 31 2009, Hits: 8732, Reviews: 0  Write review   Cached
  • SDS-PAGE Protocol (Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
    Basic-Native: For Acidic and Neutral proteins (pI <7.0)
    http://wolfson.huji.ac.il/purification/Protocols/P...
    Added: Sat Feb 21 2009, Hits: 5788, Reviews: 0  Write review   Cached
  • SDS-PAGE Protocol (Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
    Acidic-Native: For Basic proteins (pI> 7.0)
    http://wolfson.huji.ac.il/purification/Protocols/P...
    Added: Sat Feb 21 2009, Hits: 4585, Reviews: 0  Write review   Cached
  • SDS-PAGE protocol (eLabProtocols)
    Protocol to prepare and run SDS-PAGE gels
    http://www.elabprotocols.com/viewer/?id=130
    Added: Wed Sep 08 2010, Hits: 3974, Reviews: 0  Write review   Cached
  • SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE) (EnCor Biotechnology Inc.)
    http://www.encorbio.com/protocols/SDS-PAGE.htm
    Added: Sat Jan 31 2009, Hits: 6469, Reviews: 0  Write review   Cached
  • Tricine SDS-PAGE (Goldberg Lab)
    Detailed protocol
    http://wheat.pw.usda.gov/~lazo/methods/goldberg/tr...
    Added: Tue May 14 2002, Hits: 1898, Reviews: 0  Write review   Cached
  • Tricine SDS-PAGE (Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
    Tricine: SDS gel for low MW proteins (<25 kDa)
    http://wolfson.huji.ac.il/purification/Protocols/T...
    Added: Sat Feb 21 2009, Hits: 1930, Reviews: 0  Write review   Cached