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Protocols
- DNA Maxiprep Using PEG Precipitation (Sosnick Group, University of Chicago)
Need a protocol to maxiprep your plasmid with home-made recipes? This is the one you should try. Step by step guide with recipe listing.
http://sosnick.uchicago.edu/DNA_maxiprep.html
Added: Sat Mar 27 2004, Hits: 260, Reviews: 0 Write review Cached -
CsCl Maxi Prep
(Steven Finkbeiner, UCSF)
http://www.gladstone.ucsf.edu/gladstone/files/fink...
Added: Fri Feb 20 2009, Hits: 1001, Reviews: 0 Write review -
CsCl Prep. of Plasmid DNA
(Mike A. Dyer)
This is a standard large scale prep. for plasmid DNA which gives a yield of 0.5 - 1.0 mg.
http://axon.med.harvard.edu/~cepko/protocol/mike/C...
Added: Tue May 14 2002, Hits: 808, Reviews: 0 Write review Cached -
Large scale double-stranded DNA isolation
(Roe Lab)
Provides protocol of isolating double-stranded DNA from plasmid or cosmid by the alkaline lysis procedure followed by equilibrium ultracentrifugation in cesium chloride-ethidium bromide gradients and diatomaceous earth-based purification method
http://www.genome.ou.edu/protocol_book/protocol_pa...
Added: Tue May 14 2002, Hits: 808, Reviews: 0 Write review Cached -
Large Scale Plasmid Preparation
(Donis Keller Lab)
For recovery of highly purified recombinant plasmid DNA using a CsCl2 gradient.
http://wheat.pw.usda.gov/~lazo/methods/donis/plasm...
Added: Tue May 14 2002, Hits: 847, Reviews: 0 Write review Cached -
Large Scale Plasmid, Cosmid, BAC, PAC, and Fosmid DNA Isolation by a Cleared Lysate Method Followed
(Roe Lab)
http://www.genome.ou.edu/DblAcetateProcV3.html
Added: Tue May 14 2002, Hits: 571, Reviews: 0 Write review Cached -
Large-Scale Plasmid Preparation
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 265, Reviews: 0 Write review Cached -
Plasmid Maxiprep
(Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/mol...
Added: Tue May 14 2002, Hits: 410, Reviews: 0 Write review Cached