Top : Molecular Biology : Plasmid : Miniprep

Protocols

• DNA Plasmid Miniprep using Alkaline Lysis (Sosnick Group, University of Chicago)
  Detailed guide to plasmid miniprep without using a commercial kit.
  http://sosnick.uchicago.edu/DNA_miniprep.html
  Added: Sun Mar 28 2004, Hits: 1406, Reviews: 0  Write review   Cached
• Real Fast Mini Preps
  Fast miniprep without using kits
  http://www.gladstone.ucsf.edu/gladstone/files/fink...
  Added: Fri Feb 20 2009, Hits: 12268, Reviews: 0  Write review  
• "Dirty" Mini Preps (Lykke-Andersen Lab, Department of MCD Biology, University of Colorado)
  A quick miniprep method
  http://www.colorado.edu/mcdb/Lykke_Andersen/update...
  Added: Thu Feb 26 2009, Hits: 4444, Reviews: 0  Write review   Cached
• "Smash and Grab" Recovery of Plasmids (D. Amberg)
  http://www.upstate.edu/biochem/amberg/protocols/sm...
  Added: Tue May 14 2002, Hits: 1310, Reviews: 0  Write review   Cached
• Alkaline Lysis Mini Plasmid Preps (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
  Added: Tue May 14 2002, Hits: 848, Reviews: 0  Write review   Cached
• Alkaline Lysis Miniprep (Brent Graveley)
  http://penguin.uchc.edu/~intron/Protocols/Alkaline...
  Added: Tue May 14 2002, Hits: 2852, Reviews: 0  Write review   Cached
• Alkaline Lysis Minipreps of Plasmid or Cosmid DNA (Donis Keller Lab)
  Plasmid or cosmid containing E. coli cells are grown and lysed with alkali. The cell debris and chromosomal DNA is precipitated with SDS and potassium acetate. After pelleting the debris the plasmid/cosmid DNA is precipitated from the supernatant with isopropanol and the DNA resuspended in TE. The expected yields from a 5 ml culture are 5-10 ug for plasmids 2-5 ug for cosmids. The procedure can be scaled up many fold.
  http://hg.wustl.edu/hdk_lab_manual/plasmid/plsmid0...
  Added: Tue May 14 2002, Hits: 2111, Reviews: 0  Write review   Cached
• Alkaline Lysis/Phenolic Extraction of Plasmid (Plattsburgh State Univ.)
  http://faculty.plattsburgh.edu/donald.slish/extrac...
  Added: Sat Dec 08 2012, Hits: 111, Reviews: 0  Write review   Cached
• Boiling Lysis Miniprep (Brent Graveley)
  http://penguin.uchc.edu/~intron/Protocols/Boiling%...
  Added: Tue May 14 2002, Hits: 1286, Reviews: 0  Write review   Cached
• Ctab Mini Plasmid Preparation (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
  Added: Tue May 14 2002, Hits: 250, Reviews: 0  Write review   Cached
• DNA Plasmid Prep. : Baron Lab long version (Mike A Dyer)
  This is a scaled up version of the Baron protocol which has been modified to achieve purity comparable to CsCl preps.
  http://axon.med.harvard.edu/~cepko/protocol/mike/D...
  Added: Tue May 14 2002, Hits: 1192, Reviews: 0  Write review   Cached
• Mini Prep by Boiling (Howell Lab)
  http://cancer.ucsd.edu/howelllab/Mini%20Prep%20by%...
  Added: Tue May 14 2002, Hits: 1662, Reviews: 0  Write review   Cached
• Miniprep double-stranded DNA isolation (ROE)
  After collecting the plasmid containing cells by centrifugation, the cell pellet is resuspended in a hypotonic sucrose buffer. The cells are successively incubated with an RNase-lysis buffer, alkaline detergent, and sodium acetate. The lysate is cleared of precipitated proteins and membranes by centrifugation, and the plasmid DNA is recovered from the supernatant by isopropanol precipitation. The DNA is crudely checked for concentration and purity using agarose gel electrophoresis against known standards. A typical yield for this method of DNA isolation is 10-15 ug of plasmid DNA from a 6 ml starting culture.
  http://www.genome.ou.edu/protocol_book/protocol_pa...
  Added: Tue May 14 2002, Hits: 2178, Reviews: 0  Write review   Cached
• MiniPrep Protocol (Gottschling Lab, Fred Hutchinson Cancer Research Center)
  http://labs.fhcrc.org/gottschling/Bacterial%20Prot...
  Added: Thu Mar 26 2009, Hits: 3948, Reviews: 0  Write review   Cached
• PEG Preparation of Plasmid DNA (Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
  Plasmid DNA isolated by this procedure can be used routinely for electrophoretic analysis, restriction endonuclease digestion and transformation of E. Coli., DNA sequencing, PCR and most other molecular biological techniques. The procedure is a modification of the rapid alkaline lysis method of Ish-Horowitz and Burke (1981).
  http://www.nottingham.ac.uk/~mbzspd/methods/PEG_Pr...
  Added: Sat Apr 10 2004, Hits: 138, Reviews: 0  Write review   Cached
• Plasmid DNA Isolation from Bacteria (LaboratoryExperiments.com)
  Basic procedure for high school students, But very useful if you are new to molecular biology.
  http://www.laboratoryexperiments.com/LAB1.htm
  Added: Tue May 14 2002, Hits: 3498, Reviews: 0  Write review   Cached
• Plasmid DNA Minipreps (Chen)
  Included are two methods of preparing plasmid DNA, one is ammonium acetate method and the other is alternative ammonium acetate method. You can also find some useful application notes.
  http://www.biochem.ucl.ac.uk/~chen/protocols/dnapr...
  Added: Tue May 14 2002, Hits: 3086, Reviews: 0  Write review  
• Plasmid MiniPrep (Methods.info)
  Minipeps without overnight culture (8 h from colony to plasmid DNA). This method uses Qiagen solutions.
  http://www.methods.info/Methods/RNA_DNA/Plasmid_mi...
  Added: Wed Sep 04 2002, Hits: 2938, Reviews: 0  Write review   Cached
• Plasmid Miniprep (Hackett Lab, University of Minnesota)
  Mini-Prep procedure is used to isolate small plasmid DNA from bacteria while limiting contaminating proteins and genomic DNA. The plasmid quality is acceptable for restriction analysis, sequencing, cloning, or other purposes, but should not be used with out additional cleanup for embryonic injections.
  http://cbs.umn.edu/labs/perry/Protocols/mini.html
  Added: Sat Feb 28 2009, Hits: 2998, Reviews: 0  Write review   Cached
• Plasmid Minipreps (Bowtell Lab)
  http://grimwade.biochem.unimelb.edu.au/bowtell/mol...
  Added: Tue May 14 2002, Hits: 231, Reviews: 0  Write review   Cached
• Plasmid Quickpreps (Kay Schneitz Lab)
  Nice protoocl for preparing plasmid DNA from a single colony
  http://www.unizh.ch/botinst/Cyto_Website/schneitzL...
  Added: Tue May 14 2002, Hits: 1407, Reviews: 0  Write review   Cached
• Preparing Overnight Bacteria Culture (LaboratoryExperiments.com)
  This is a basic procedure for high school students and useful for those who are new to molecular biology.
  http://www.laboratoryexperiments.com/LAB5.htm
  Added: Tue May 14 2002, Hits: 985, Reviews: 0  Write review   Cached
• Quick DNA Plasmid Prep. : Boiling 10 minute protocol (Mike A Dyer)
  This is a very fast mini-prep protocol which is suitable for sequence analysis and restriction digests. Although the yield is higher, there is considerable chromosomal DNA, RNA and protein contamination.
  http://axon.med.harvard.edu/~cepko/protocol/mike/D...
  Added: Tue May 14 2002, Hits: 892, Reviews: 0  Write review   Cached
• Quick Plasmid DNA Preparation (Mike A Dyer)
  This protocol gives very clean plasmid preps for restriction digests and cloning. However, due to the alkaline lysis step, the DNA is often nicked and may not give exceptional sequence data.
  http://axon.med.harvard.edu/~cepko/protocol/mike/D...
  Added: Tue May 14 2002, Hits: 1117, Reviews: 0  Write review   Cached
• Silica-based Plasmid Miniprep (Dr. Ian Chin-Sang)
  Silica-based Plasmid Miniprep with vacuum manifold.
  http://research.abl.es/methods/1426/
  Added: Fri Feb 17 2012, Hits: 218, Reviews: 0  Write review  

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