Protocols
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Small-Scale Purification of Plasmid
(Hideaki Shiraishi, Kyoto University)
Detailed procedures with recipes.
http://kuchem.kyoto-u.ac.jp/seika/shiraishi/protoc...
Added: Fri Jan 30 2004, Hits: 1703, Reviews: 0 Write review Cached -
Standard (alkaline lysis) Mini-Prep
(Goldberg Lab)
Standard protocol for mini-prep and recipe for solution I, II and III.
http://wheat.pw.usda.gov/~lazo/methods/goldberg/mi...
Added: Tue May 14 2002, Hits: 1403, Reviews: 0 Write review Cached -
Toothpick Plasmid Assay
(Donis Keller Lab)
The toothpick assay is used to identify bacterial colonies containing recombinant plasmids following transformation and to obtain an approximation of the plasmid insert sizes.
http://hg.wustl.edu/hdk_lab_manual/plasmid/plsmid0...
Added: Tue May 14 2002, Hits: 646, Reviews: 0 Write review Cached