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Protocols
- Plasmid Isolation Methods (Bruce A. Roe Lab, University of Oklahoma)
A ensemble of protocols on plasmid DNA isolation including Large scale double-stranded DNA isolation, Midiprep double-stranded DNA isolation, Miniprep double-stranded DNA isolation, Large scale M13RF isolation, Single-stranded M13 DNA isolation using phenol, Biomek-automated modified-Eperon isolation procedure for single-stranded M13 DNA
http://www.genome.ou.edu/protocol_book/protocol_pa...
Added: Tue Feb 03 2009, Hits: 7087, Reviews: 0 Write review Cached -
Extrachromosomal Elements-Plasmids
(Michael Blaber)
What's plasmid? Find answer here. It provides background information on the features of plasmid, selection marker and more...
http://wine1.sb.fsu.edu/bch5425/lect13/lect13.htm
Added: Tue May 14 2002, Hits: 332, Reviews: 0 Write review Cached -
PCR Amplification of Inserts from Bacterial Cultures
(CIMMYT Applied Molecular Genetics Laboratory)
PCR amplification of plasmid insert without plasmid preparation
http://www.cimmyt.org/english/docs/manual/protocol...
Added: Mon Aug 05 2002, Hits: 3944, Reviews: 0 Write review -
Plasmid stability test
(The Protein Expression and Purification Facility, EMBL)
Immediately before indcution, it is advisable to test the culture to determine the fraction of cells that still carry the target plasmid. This involves plating of cells on four different plates.
http://www.embl-heidelberg.de/ExternalInfo/geerlof...
Added: Sat Sep 21 2002, Hits: 1837, Reviews: 0 Write review Cached -
Recovery plasmids from dead bacterial
(Nonet Lab, Washington University in St. Louis)
Method to recover plasmids from dead bacteria stored at -80 C
http://thalamus.wustl.edu/nonetlab/ResourcesF/plas...
Added: Thu Feb 26 2009, Hits: 1426, Reviews: 0 Write review Cached