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- DNA PAGE Gel Electrophoresis (School of Chem. and Biochem, Georgia Institutue of Technology)
Nucleic acid PAGE electrophoresis utilizes the same concepts as protein electrophoresis. The denaturant and visualization techniques differ. All nucleic acids are negatively charged. It is not neccessary or desirable to use a charged denaturant such as SDS. Urea is used to denature the DNA or RNA within the gel. Visualization is usually achieved via staining with ethidium bromide...
http://ww2.chemistry.gatech.edu/~lw26/bCourse_Info...
Added: Sat Aug 04 2012, Hits: 2136, Reviews: 0 Write review - Preparation of Polyacrylamide Gels (John Roth, Division of Biological Sciences,University of California at Davis)
Detailed procedure and receipes
http://rothlab.ucdavis.edu/protocols/Z03.html
Added: Tue May 25 2004, Hits: 10765, Reviews: 0 Write review Cached -
Linear Gradient Gels
(John Garland, The Biochemistry and Molecular Biology Department of The James H. Quillen College of Medicine in Johnson City, TN)
Method for preparation of SDS-PAGE Gel
http://qcom.etsu.edu/biochem/protocols/linear.htm
Added: Sat Sep 14 2002, Hits: 494, Reviews: 0 Write review Cached -
NuPAGE Gel Electrophoresis
(Kitto Lab, The University of Texas at Austin)
A gel electrophoresis system used for SDS-PAGE protein analysis. The gels are made up of Bis-Tris-HCl (pH 6.4) polyacrylamide and are intended for denaturing conditions only.
http://kitto.cm.utexas.edu/research/Kittolabpage/P...
Added: Tue Oct 08 2002, Hits: 1785, Reviews: 0 Write review Cached -
PolyAcrylamide Gel Electrophoresis (PAGE) Gels
(John Garland, The Biochemistry and Molecular Biology Department of The James H. Quillen College of Medicine in Johnson City, TN)
Preparation of stock solutions of acrylamide and method of preparation of convex, and gradient gels
http://qcom.etsu.edu/biochem/protocols/gels.htm
Added: Tue May 14 2002, Hits: 1644, Reviews: 0 Write review Cached -
Polyacrylamide Gel Electrophoresis of Oligonucleotides
(John Roth, Division of Biological Sciences,University of California at Davis)
http://rothlab.ucdavis.edu/protocols/E11.html
Added: Sun Jun 06 2004, Hits: 4033, Reviews: 0 Write review Cached