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Protocols
- Agarose-Formaldehyde Electrophoresis (Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
RNA electrophoresis under denaturing conditions in 2.2M formaldehyde is performed according to Maniatis et al., (1982) using the MOPS buffer system. RNA under these conditions is fully denatured and migrates according to the log10 of its molecular weight.
http://www.nottingham.ac.uk/~mbzspd/methods/Agaros...
Added: Sat Apr 10 2004, Hits: 7737, Reviews: 0 Write review Cached - Denaturing and Nature Agarose Gel Electrophoresis of RNA (Ambion)
Two general methods are described: denaturing agarose gel and native agarose gel electrophoresis.
http://www.ambion.com/techlib/append/supp/rna_gel....
Added: Sun Jul 30 2006, Hits: 6660, Reviews: 0 Write review Cached -
Denaturing polyacrylamide gel electrophoresis of RNA
(Beverly Faulkner-Jones)
Polyacrylamide gels have enough resolving power to separate fragments differing by only one base pair in size, but their range is ~ 5 to 1000 bp. They are much more difficult to handle than agarose gels.
http://grimwade.biochem.unimelb.edu.au/bfjones/gen...
Added: Tue May 14 2002, Hits: 4128, Reviews: 0 Write review Cached -
Formaldehyde-Agarose Gels
(Beverly Faulkner-Jones)
Agarose gels have a smaller resolving power than polyacrylamide gels but a greater range of separation - from 200 bp to >50 kb using standard gels and electrophoresis equipment. RNAs up to 10 000 kb can be separated in agarose gels using pulsed field gel electrophoresis.
http://grimwade.biochem.unimelb.edu.au/bfjones/gen...
Added: Tue May 14 2002, Hits: 3964, Reviews: 0 Write review Cached -
Gel Electrophoresis of RNA
(Beverly Faulkner-Jones)
great tips on RNA gel electrophoresis.
http://grimwade.biochem.unimelb.edu.au/bfjones/gen...
Added: Tue May 14 2002, Hits: 7594, Reviews: 0 Write review Cached -
Glyoxal Gel Electorphoresis of RNA
(Karl Clark, University of Minnesota)
Glyoxal/DMSO gels are great for determining sizes of RNA and can be used with Northern blotting
http://www.cbs.umn.edu/~kclark/protocols/glyoxal.h...
Added: Sun Jul 30 2006, Hits: 642, Reviews: 0 Write review Cached -
Polyacrylamide Gel Electrophoresis (PAGE) of RNA
(Frank Bottone)
For use withRibonuclease Protection Assay (RPA):
http://www.unc.edu/~fbottone/protocol/page.html
Added: Tue May 14 2002, Hits: 5439, Reviews: 0 Write review Cached -
Recovery of RNA fragments from polyacrylamide gels
(Beverly Faulkner-Jones)
The best method is the crush and soak method. It can be sued to isolate single-stranded RNA from denaturing gels. The RNA is of high purity but the method is long and inefficient if long transcripts are to be recovered (cf. less than 30% of DNA greater than 3 kb is recovered).
http://grimwade.biochem.unimelb.edu.au/bfjones/gen...
Added: Tue May 14 2002, Hits: 1424, Reviews: 0 Write review Cached