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  • Generation of Uni-directional Nested Deletions (Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
    Making uni-directional nested deletion in plasmid DNA.
    http://www.nottingham.ac.uk/~mbzspd/methods/Genera...
    Added: Sat Apr 10 2004, Hits: 1433, Reviews: 0  Write review   Cached
  • Theory, principles and background info In vitro Site-Specific Mutagenesis (NCBI)
    mutagenesis can be conducted in vivo (in studies of model organisms, or cultured cells) or in vitro and the mutagenesis can be directed to a specific site in a pre-determined way (site-directed mutagenesis), or can be random. This book chapter introduces different mutagenesis methods.
    http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=hmg....
    Added: Sat Jun 12 2004, Hits: 8770, Reviews: 0  Write review   Cached
  • "Round-the-Horn" Site-Directed Mutagenesis (OpenWetWare)
    'Round-the-horn is a PCR-based mutagenesis. The muations are contained in one or both of the primers. The primers are phosphorylated so that the PCR product can be ligated into a circle and used to transform cells. The procedure has the advantages that the primers are smaller, a visible product on a gel means your reaction worked and must contain the encoded mutations, only one primer needs to be changed to change the mutation at the same site...
    http://openwetware.org/wiki/%27Round-the-horn_site...
    Added: Sun Mar 04 2007, Hits: 960, Reviews: 0  Write review   Cached
  • Creating a Seletion by PCR Splicing (Methods.info)
    To delete a desired fragment from existing DNA fragment all you need is a pair of primers combined of flanking sequences and a high fidelity polymerase.
    http://www.methods.info/Tips/mutagenesis/PCR_splic...
    Added: Sat Dec 20 2003, Hits: 1590, Reviews: 0  Write review   Cached
  • Deletion Mutagenesis (Promega)
    For construction of plasmid or M13 subclones containing progressive unidirectional deletions of inserted DNA, Including methods for DNA preparation, vector digestion, exonuclease deletion, ligation, transformation...
    http://www.promega.com/tbs/tm006/tm006.html#i
    Added: Tue May 14 2002, Hits: 3117, Reviews: 0  Write review   Cached
  • DpnI Mediated Site-Directed Mutagenesis (Nonet Lab - Washington University School of Medicine)
    A highly effective simple method for making site directed lesions in plasmids without subcloning.
    http://thalamus.wustl.edu/nonetlab/ResourcesF/dpnm...
    Added: Sat Nov 01 2003, Hits: 2733, Reviews: 0  Write review   Cached
  • Dut/ung in vitro Site-directed Mutagenesis PDF file (Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
    Detailed procedures
    http://www.its.caltech.edu/%7Ebjorker/Site-directe...
    Added: Wed Aug 14 2002, Hits: 1692, Reviews: 0  Write review  
  • Exo/S1 Deletion Series (Vesicle Trafficking)
    http://cmgm.stanford.edu/~vesicle/protocols/exo_s1...
    Added: Tue May 14 2002, Hits: 529, Reviews: 0  Write review   Cached
  • ExoIII/S1 Deletion (Fermentas)
    For construction of plasmid or M13 subclones with nested unidirectional deletions. The method is applicable for DNA sequencing, mapping of boundaries of regions involved in genetic control, DNA/protein interaction sites, or protein domains.
    http://www.fermentas.com/Profiles/Kits/PExoIIIKit....
    Added: Tue May 14 2002, Hits: 914, Reviews: 0  Write review   Cached
  • In vitro Mutagenesis Using Altered Sites (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
    http://www.pmci.unimelb.edu.au/core_facilities/man...
    Added: Tue May 14 2002, Hits: 964, Reviews: 0  Write review   Cached
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