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Creating a deletion by PCR splicing

Contributed by Dr.A.Gratchev

I didn't want to place this in the methods section due to its simplicity. To delete a desired fragment from existing DNA fragment all you need is a pair of primers combined of flanking sequences and a high fidelity polymerase (a mix of Taq and Pfu for example). The procedure is shown on the picture. The only notes which I want to make are: