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Isolation of DNA from agarose gels
M.9: ALTERNATE METHOD FOR PURIFYING DNA FROM AGAROSE GELS
1. Excise band of interest from a TAE gel; estimate volume by weighing the gel
slice.
2. Dissolve the gel slice in 3 volumes of NaI (from Geneclean kit) at 55oC for 5
min or until the gel dissolves.
3. Add 1 ml of Wizard miniprep resin (Promega), 10 min. at room temperature.
4. Add DNA resin mixture to a syringe and pass through a minicolumn.
5. Wash with 2 ml of Wizard wash buffer; spin 2 min in a microfuge.
6. Elute with 70oC TE or water (50 ul), 2 min in a microfuge.
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