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Protocols
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Immunofluorescence Labeling of Cells
(Sigma-Aldrich Corporation)
Complete protocol detailing the four steps of cell staining: cell preparation, fixation, application of antibody, and evaluation.
http://www.sigmaaldrich.com/Area_of_Interest/Life_...
Added: Fri Jul 11 2008, Hits: 89, Reviews: 0 Write review -
Immunofluorescence Staining and Flow Cytometry of Intracellular Cytokines
(eBioscience)
A modification of the basic immunofluorescence staining and flow cytometric analysis protocol can be used for the simultaneous analysis of surface molecules and intracellular cytokines at single-cell level. In this protocol, cells are first activated in vitro, stained for surface antigens, as in the regular staining protocol, then fixed and permeabilized to allow for anti-cytokine antibodies to stain intracellularly.
http://www.ebioscience.com/ebioscience/appls/FCI.h...
Added: Tue May 14 2002, Hits: 1371, Reviews: 0 Write review Cached -
Immunofluorescence Staining of Cells on Coverslips
(Cooper Lab, Department of Cell Biology & Physiology Washington University in St Louis)
Cells are grown on coverslips, fixed and stained with primary and secondary antibody
http://www.cooperlab.wustl.edu/Methods/Immunostain...
Added: Mon Aug 14 2006, Hits: 965, Reviews: 0 Write review -
In Situ Hybridization of mRNA and Immunofluorescence
(Corbett Lab, Emory University)
Yeast protocol
http://www.biochem.emory.edu/labs/acorbe2//protoco...
Added: Tue May 14 2002, Hits: 620, Reviews: 0 Write review Cached -
Minimizing Background Staining in immunofluorescence Procedures
(The Janis V. Giorgi Flow Cytometry Laboratory, UCLA)
Tips on reducing background staining
http://cyto.mednet.ucla.edu/Protocols/minimizi.htm
Added: Sat Feb 11 2006, Hits: 731, Reviews: 0 Write review Cached -
Preparation of cytospin from single cell suspension
(Giorgio Cattoretti, Columbia University )
http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
Added: Fri Aug 30 2002, Hits: 1955, Reviews: 0 Write review Cached -
Preparation of Mowiol Mounting Medium
(Stephens Lab, University of Bristol)
http://www.bristol.ac.uk/biochemistry/stephens/MOW...
Added: Sat Mar 11 2006, Hits: 67, Reviews: 0 Write review Cached -
Preparing a Cell Block from Cell Lines/Cell Suspensions
(Giorgio Cattoretti, Columbia University )
http://icg.cpmc.columbia.edu/cattoretti/Protocol/I...
Added: Fri Aug 30 2002, Hits: 681, Reviews: 0 Write review Cached -
Progesterone receptor/BrdU double Immunofluorescence
(Rosen Lab, Department of Molecular and Cellular Biology, Baylor College of Medicine)
http://www.bcm.edu/rosenlab/protocols/PR_BrdU.pdf
Added: Sat Aug 24 2002, Hits: 1283, Reviews: 0 Write review -
Staining for Surface Receptors by Feeding Antibody
(Von Zastrow lab)
This protocols allows the use of fluorescence microscopy to visualize epitope-tagged receptors expressed by stable adherent cell lines. In contrast to the permeabalized cell protocol, this protocol where antibody is "fed" to live unpermeabalized cells will only visualize receptors that started out on the surface of the cell.
http://www.ucsf.edu/vonzast/feeding.html
Added: Tue May 14 2002, Hits: 878, Reviews: 0 Write review Cached