Protocols
- Direct Immunofluorescence Staining of Cells (The Science Advisory Board)
This is the method for direct immunofluorescence labeling; that is, the antibodies have the fluorescent dye attached. Direct labeling is simpler and quicker than indirect labeling.
http://www.scienceboard.net/resources/protocols.as...
Added: Mon Feb 23 2009, Hits: 19537, Reviews: 0 Write review Cached -
Direct Immunofluorescence Staining
(MRC Centre for Regenerative Medicine, UK)
Staining cells with antibodies directly linked to fluorochromes is know as direct immunofluorescence (DIF). DIF lends itself to multicolour experiments where a cell suspension is simultaneously stained with two, three or four antibodies each tagged with a different fluorescent dye.
http://www.crm.ed.ac.uk/facilities/flow-cytometry/...
Added: Mon Feb 23 2009, Hits: 9683, Reviews: 0 Write review Cached