Immunofluorescence/Direct Immunofluorescence Staining Protocols

Direct Immunofluorescence Staining of Cells (The Science Advisory Board)
This is the method for direct immunofluorescence labeling; that is, the antibodies have the fluorescent dye attached. Direct labeling is simpler and quicker than indirect labeling.
http://www.scienceboard.net/resources/protocols.as...
Added: Mon Feb 23 2009, Hits: 13425, Reviews: 0 Write review Cached
Direct Immunofluorescence Staining (MRC Centre for Regenerative Medicine, UK)
Staining cells with antibodies directly linked to fluorochromes is know as direct immunofluorescence (DIF). DIF lends itself to multicolour experiments where a cell suspension is simultaneously stained with two, three or four antibodies each tagged with a different fluorescent dye.
http://www.crm.ed.ac.uk/facilities/flow-cytometry/...
Added: Mon Feb 23 2009, Hits: 6255, Reviews: 0 Write review Cached