Protocols
- Immunostaining of Cells in Tissue Culture (EnCor Biotechnology)
Procedure for immunofluorescence staining of cultured cells.
http://www.encorbio.com/protocols/immunotc.htm
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Immunofluoresence Staining protocol for perfusion fixed tissue
(Abcam)
recommended for rat tissue fixed by paraformaldehyde-picric acid perfusion
http://www.abcam.com/index.html?rid=10188
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Immunofluorescence of Cells
(The Morimoto Lab, Northwestern University)
This protocol is used for staining cells grown in 2-well glass chamber.
http://www.biochem.northwestern.edu/morimoto/resea...
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Immunofluorescence of Parafilm Sections
(Chen Lab, UCSF)
http://bts.ucsf.edu/chen/Protocol/Parafilm%20Secti...
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Immunofluorescence Protocols and Solutions
(Salmon Lab, University of North Carolina at Chapel Hill, Department, of Biology)
http://www.bio.unc.edu/faculty/salmon/lab/protocol...
Added: Tue May 14 2002, Hits: 10295, Reviews: 0 Write review Cached -
Immunofluorescence Staining and Flow Cytometry of Intracellular Cytokines
(eBioscience)
A modification of the basic immunofluorescence staining and flow cytometric analysis protocol can be used for the simultaneous analysis of surface molecules and intracellular cytokines at single-cell level. In this protocol, cells are first activated in vitro, stained for surface antigens, as in the regular staining protocol, then fixed and permeabilized to allow for anti-cytokine antibodies to stain intracellularly.
http://www.ebioscience.com/ebioscience/appls/FCI.h...
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Immunofluoresence Staining with Parafilm Section Slide (Double Staining)
(Chen Lab, UCSF)
http://bts.ucsf.edu/chen/Protocol/Parafilm%20Secti...
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Immunostaining of tissue sections
(EnCor Biotechnology)
Here are two alternative procedures, both of which work well. The more well established method is to perfuse the animal with fixative while under anesthetic. It is a lot easier to sacrifice the animal first, dissect out the tissues you want and just drop them into fixative. Fixed tissues are then impregnated with sucrose, which acts as a cryoprotectant, preventing ice crystal formation during freezing. Specimens are then rapidly frozen and sectioned on a cryostat.
http://www.encorbio.com/protocols/immunots.htm
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Indirect Immunofluorescence Staining
(U of Florida Flow Cytometry Core Laboratory)
This is the method for indirect immunofluorescence labeling; that is, the antibodies do not have the fluorescent dye attached.
http://www.biotech.ufl.edu/FlowCytometry/indirect....
Added: Tue May 14 2002, Hits: 1251, Reviews: 0 Write review Cached