Protocols
- Immunofluorescence on Cells (Dr. Walter Steffen,MRC Muscle and Cell Motility Unit, King's College London)
Immunofluorescence staining of cultured cells including various methods of fixation, staining.
http://www.kcl.ac.uk/depsta/biomedical/randall/met...
Added: Tue Aug 10 2004, Hits: 21769, Reviews: 0 Write review Cached - Immunofluorescence Protocols and Solutions (Salmon Lab, Department of Biology, University of North Carolina at Chapel Hill)
Procedure include making boiled donkey (or whatever) serum (BDS) and heat-inactivated serum for blocking, cell fixation and staining.
http://www.bio.unc.edu/faculty/salmon/lab/protocol...
Added: Tue May 14 2002, Hits: 12703, Reviews: 0 Write review Cached - Immunostaining of Cells in Tissue Culture (EnCor Biotechnology)
Procedure for immunofluorescence staining of cultured cells.
http://www.encorbio.com/protocols/immunotc.htm
Added: Sat Jul 30 2005, Hits: 8824, Reviews: 0 Write review Cached -
IF Protocol for Perfusion Fixed Tissue
(Abcam Ltd)
recommended for rat tissue fixed by paraformaldehyde-picric acid perfusion
http://www.abcam.com/index.html?rid=10188
Added: Mon May 23 2005, Hits: 3516, Reviews: 0 Write review Cached -
Immunfluoresence on Chamber Slides
(Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
Added: Tue May 14 2002, Hits: 2537, Reviews: 0 Write review Cached -
Immunofluorescence
(Corbett Lab)
Yeast protocol
http://www.biochem.emory.edu/labs/acorbe2/protocol...
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Immunofluorescence (without methanol)
(D. Amberg)
http://www.upstate.edu/biochem/amberg/protocols/no...
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Immunofluorescence of Cells
(The Morimoto Lab, Northwestern University)
This protocol is used for staining cells grown in 2-well glass chamber.
http://www.biochem.northwestern.edu/ibis/morimoto/...
Added: Mon Mar 29 2004, Hits: 6927, Reviews: 0 Write review -
Immunofluorescence Staining of Cells
(Spector Lab, CSHL)
protocol for immunofluorescence on cells
http://spectorlab.cshl.edu/immunofluorescence.html
Added: Tue May 14 2002, Hits: 9277, Reviews: 0 Write review Cached -
Immunofluorescence Staining of Tissue Specimen
(EnCor Biotechnology)
Here are two alternative procedures, both of which work well. The more well established method is to perfuse the animal with fixative while under anesthetic. It is a lot easier to sacrifice the animal first, dissect out the tissues you want and just drop them into fixative. Fixed tissues are then impregnated with sucrose, which acts as a cryoprotectant, preventing ice crystal formation during freezing. Specimens are then rapidly frozen and sectioned on a cryostat.
http://www.encorbio.com/protocols/immunots.htm
Added: Sat Jul 30 2005, Hits: 4795, Reviews: 0 Write review Cached