Top : Immunology : Immunofluorescence : Indirect Immunofluoresence Staining
Sponsored Links:
Protocols
  • Immunostaining of Cells in Tissue Culture (EnCor Biotechnology)
    Procedure for immunofluorescence staining of cultured cells.
    http://www.encorbio.com/protocols/immunotc.htm
    Added: Mon Feb 23 2009, Hits: 8973, Reviews: 0  Write review   Cached
  • Immunofluoresence Staining protocol for perfusion fixed tissue (Abcam)
    recommended for rat tissue fixed by paraformaldehyde-picric acid perfusion
    http://www.abcam.com/index.html?rid=10188
    Added: Mon Feb 23 2009, Hits: 3508, Reviews: 0  Write review   Cached
  • Immunofluorescence of Cells PDF file (The Morimoto Lab, Northwestern University)
    This protocol is used for staining cells grown in 2-well glass chamber.
    http://www.biochem.northwestern.edu/morimoto/resea...
    Added: Mon Feb 23 2009, Hits: 8114, Reviews: 0  Write review  
  • Immunofluorescence of Parafilm Sections PDF file (Chen Lab, UCSF)

    http://bts.ucsf.edu/chen/Protocol/Parafilm%20Secti...
    Added: Sun Oct 25 2009, Hits: 2110, Reviews: 0  Write review  
  • Immunofluorescence Protocols and Solutions (Salmon Lab, University of North Carolina at Chapel Hill, Department, of Biology)
    http://www.bio.unc.edu/faculty/salmon/lab/protocol...
    Added: Tue May 14 2002, Hits: 7439, Reviews: 0  Write review   Cached
  • Immunofluorescence Staining and Flow Cytometry of Intracellular Cytokines (eBioscience)
    A modification of the basic immunofluorescence staining and flow cytometric analysis protocol can be used for the simultaneous analysis of surface molecules and intracellular cytokines at single-cell level. In this protocol, cells are first activated in vitro, stained for surface antigens, as in the regular staining protocol, then fixed and permeabilized to allow for anti-cytokine antibodies to stain intracellularly.
    http://www.ebioscience.com/ebioscience/appls/FCI.h...
    Added: Tue May 14 2002, Hits: 1792, Reviews: 0  Write review   Cached
  • Immunofluoresence Staining with Parafilm Section Slide (Double Staining) PDF file (Chen Lab, UCSF)
    http://bts.ucsf.edu/chen/Protocol/Parafilm%20Secti...
    Added: Sun Oct 25 2009, Hits: 1582, Reviews: 0  Write review  
  • Immunostaining of tissue sections (EnCor Biotechnology)
    Here are two alternative procedures, both of which work well. The more well established method is to perfuse the animal with fixative while under anesthetic. It is a lot easier to sacrifice the animal first, dissect out the tissues you want and just drop them into fixative. Fixed tissues are then impregnated with sucrose, which acts as a cryoprotectant, preventing ice crystal formation during freezing. Specimens are then rapidly frozen and sectioned on a cryostat.
    http://www.encorbio.com/protocols/immunots.htm
    Added: Mon Feb 23 2009, Hits: 3497, Reviews: 0  Write review   Cached
  • Indirect Immunofluorescence Staining (U of Florida Flow Cytometry Core Laboratory)
    This is the method for indirect immunofluorescence labeling; that is, the antibodies do not have the fluorescent dye attached.
    http://www.biotech.ufl.edu/FlowCytometry/indirect....
    Added: Tue May 14 2002, Hits: 916, Reviews: 0  Write review   Cached