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• Molecular Biology/DNA/DNA Extraction & Purification

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Protocols

• BAC DNA Purification For Injection (Case Transgenic and Targeting Facility)
  Restrict DNA, gel purification, ethanol precipitation...
  http://transgenic.cwru.edu/protocols/bacpurificati...
  Added: Tue May 14 2002, Hits: 270, Reviews: 0  Write review   Cached
• Preparing Vector DNA For Transfection/Targeting (Case Transgenic and Targeting Facility)
  Highly purified DNA is important for high rates of targeting and transfection. This protocol describes procedures for plasmid prep,Cutting And Purifying DNA For Transfection/Targeting, and Prepping DNA For Transfection Of Circular DNA
  http://transgenic.cwru.edu/protocols/DavesNotes3.h...
  Added: Sun Mar 22 2009, Hits: 618, Reviews: 0  Write review   Cached
• BAC DNA Purification for Microinjection (University of Michigan, Transgenic Animal Model Core)
  This method is used to purify BAC DNA for microinjection into fertilized mouse eggs. This method yields clean, non-sheared DNA
  http://www.med.umich.edu/tamc/BACDNA.html
  Added: Tue May 14 2002, Hits: 368, Reviews: 0  Write review   Cached
• DNA Preparation for microinjection or electroporation (Transgenic and ES Cell Services at OSU)
  http://www.med.ohio-state.edu/tafhome/webpages/pro...
  Added: Tue May 14 2002, Hits: 150, Reviews: 0  Write review   Cached
• DNA Purification for Electroporation (University of Michigan, Transgenic Animal Model Core)
  http://www.med.umich.edu/tamc/electro.html
  Added: Tue May 14 2002, Hits: 220, Reviews: 0  Write review   Cached
• DNA Purification for Microinjection (University of Michigan, Transgenic Animal Model Core)
  http://www.med.umich.edu/tamc/injDNA.html
  Added: Tue May 14 2002, Hits: 218, Reviews: 0  Write review   Cached
• DNA Purification for Transgene Microinjection (U. of Virginia Transgenic Mouse Core Facility)
  http://www.med.virginia.edu/medicine/inter-dis/tra...
  Added: Tue May 14 2002, Hits: 192, Reviews: 0  Write review   Cached
• DNA Ultrapurification for Microinjection (Gary A. J. Brown)
  Several methods are described in detail.
  http://ourworld.compuserve.com/homepages/TheBroons...
  Added: Tue May 14 2002, Hits: 126, Reviews: 0  Write review   Cached
• Isolation of genomic DNA from tail biopsies to check for transgenic founder animals (Practical Approach Online, Oxford University Press)
  http://www.oup.co.uk/pdf/pas/13-9-8.pdf
  Added: Wed Sep 04 2002, Hits: 155, Reviews: 0  Write review  
• Isolation of linearized BAC DNA for microinjection to produce transgenic animals (Practical Approach Online, Oxford University Press)
  http://www.oup.co.uk/pdf/pas/13-9-3.pdf
  Added: Wed Sep 04 2002, Hits: 175, Reviews: 0  Write review  
• Isolation of linearized plasmid DNA constructs for microinjection to produce transgenic animals (Practical Approach Online, Oxford University Press)
  http://www.oup.co.uk/pdf/pas/13-9-1.pdf
  Added: Wed Sep 04 2002, Hits: 198, Reviews: 0  Write review  
• Isolation of YAC DNA for microinjection to produce transgenic animals (Practical Approach Online, Oxford University Press)
  http://www.oup.co.uk/pdf/pas/13-9-2.pdf
  Added: Wed Sep 04 2002, Hits: 101, Reviews: 0  Write review  
• Preparation of Transgene DNA for Microinjection (New England Medical Center)
  http://160.109.101.132/mcri/CoreFacilities/NEMCTUS...
  Added: Tue May 14 2002, Hits: 211, Reviews: 0  Write review   Cached
• Sample Preparation for Microinjection (Brinkmann)
  Microinjection is the loading or transfer of a dissolved substance into a living cell. The microscopic tip of the glass microcapillary has an inner diameter between 0.2 and 1 µm [1]. This capillary is back loaded with the substance to be transferred into the cells cultured for microinjection. Typical substances include purified antibodies, DNA, RNA, peptides, or oligonucleotides.
  http://www.brinkmann.com/ECET_appl2.asp
  Added: Tue May 14 2002, Hits: 76, Reviews: 0  Write review   Cached