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Overexpression Related Discussions
  1. Does over-expression protein under T7 promoter in Top10 work? - (reply: 1)
  2. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
  3. Can the pcDNA5/FRT/TO vector be used for transient overexpression? - (reply: 2)
  4. miRNA overexpression troubleshooting - (reply: 2)
  5. overexpress mature microRNA by siRNA vector - (reply: 2)
  6. Cell lines selected with G418 but no overexpression of GOI - (reply: 3)
  7. pcDNA overexpression - (reply: 4)
  8. Is overexpression a correct way to check shRNA knockdown efficiency? - (reply: 9)
  9. What is the best way to overexpress miRNAs? - (reply: 1)
  10. Overexpressed protein shows a band higher than expected?! - (reply: 2)
  11. gene overexpression in cells: virus transduction or transfection following selec - (reply: 4)
  12. Can I use custom made synthetic miRNA for Overexpression? - (reply: 1)
  13. overexpression using pcDNA3.0 in stably knockdown cells - (reply: 3)
  14. experimental setting question (overexpression control) - (reply: 2)
  15. RNAi in combination with adenoviral-mediated over-expression - (reply: 1)
  16. Bacterial expression: incorporating radioactivity into overexpressed protein - (reply: 1)
  17. microarray after overexpression and silencing - (reply: 2)
  18. overexpression FLAG-tagged protein shows extra bands in WB - (reply: 2)
  19. Forcing overexpression of a GOI - (reply: 7)
  20. apoptosis upon over-expression with pcDNA6-cDNA - how prove sequence specificit - (reply: 4)
  21. Overexpression plasmid is not expressing protein - (reply: 13)
  22. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
  23. C-terminal Cleavage During Overexpression - (reply: 3)
  24. protein overexpression in HeLa cells - (reply: 1)
  25. 2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
  26. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
  27. How to overexpress miRs - (reply: 1)
  28. miRNA overexpression for target validation - (reply: 1)
  29. protein overexpression - factors affecting overexpression (reply: 3)
  30. transcription factor overexpression - (reply: 3)
  31. Protein Degradation after overexpression - (reply: 1)
  32. Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
  33. overexpression of stable clones - (reply: 1)
  34. A large proportion of mislocalization of my overexpressed GFP fused protein - Any suggestion please? (reply: 7)
  35. lentiviral overexpression of miRNAs - (reply: 6)
  36. Loading Equal protein concentrations - Does it make a difference in simple overexpression checking? (reply: 4)
  37. Trouble about overexpression by lentiviral transduction - Why no protein of interest expressed!? (reply: 1)
  38. Overexpressed protein not detected - Oh, the frustration... (reply: 1)
  39. Storage of overexpressed protein - (reply: 4)
  40. How to overexpress star miRNA (miRNA*) from pre-miRNA construct - (reply: 4)
  41. Overexpression experiment controls - (reply: 2)
  42. Problem with stable overexpression cell lines - (reply: 3)
  43. Antibody Characterization - KO vs. WT or Heterologous overexpression (reply: 8)
  44. protein overexpression question. - (reply: 4)