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RT-qPCR Related Discussions
  1. Contamination in qRT-PCR RT-minus - band similar to cDNA, not sure what it is? - (reply: 1)
  2. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 6)
  3. different amount of cDNA in qRT-PCR for target and reference genes - (reply: 3)
  4. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
  5. qRT-PCR works one day, and not the next - (reply: 1)
  6. Inter run calibration qRT-PCR - (reply: 2)
  7. ABI 7300 qRT-PCR and ddCT protocol - (reply: 1)
  8. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
  9. RT-qPCR primer problem - (reply: 4)
  10. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
  11. High GC content RT-qPCR - please help - (reply: 3)
  12. qRT-PCR data analysis - (reply: 2)
  13. RT-qPCR normalization - (reply: 3)
  14. What is a use of ΔCt and ΔΔCt in qRT PCR? - (reply: 4)
  15. RT-qPCR plate set up NoRT controls - (reply: 1)
  16. Experiment plan for RT-qPCR internal reference search - (reply: 3)
  17. Experimental set up for qRT PCR - (reply: 13)
  18. Is some amount of RNA obligatory for qRT-PCR? - (reply: 5)
  19. Very late CP for control in rt-qPCR from RNAlater samples - (reply: 3)
  20. real time qRT-PCR and low expression gene - (reply: 2)
  21. Sybr green RT-qPCR primer - (reply: 1)
  22. Sybr green RT-qPCR primer - (reply: 1)
  23. Improving qRT-PCR detection limits - (reply: 2)
  24. Sequencing miRNA qRT-PCR Products - (reply: 5)
  25. Using DNA as a reference in qRT-PCR - (reply: 1)
  26. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
  27. negative control in qRT-PCR - (reply: 6)
  28. RT-qPCR Experimental Design - How to arrange experimental replicates for treatment and control (reply: 1)
  29. Questions of Standards making in relative qRT-PCR - (reply: 2)
  30. relative comparison of many samples and genes in qRT-PCR - (reply: 1)
  31. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
  32. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
  33. PCR analysis method- delta or delta delta? - RNA, qRT PCR, delta Ct method (reply: 4)
  34. a question about 16s rRNA as reference in RT-qPCR - (reply: 1)
  35. question about qRT-PCR preparation - (reply: 1)
  36. General qRT-PCR question - (reply: 3)
  37. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
  38. Relative gene expression - Newbie at RT-qPCR! (reply: 3)
  39. qRT-PCR Inconsistency - (reply: 1)
  40. Workflow of qRT-PCR for siRNA - help please. (reply: 5)
  41. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
  42. qRT-PCR Product Size - (reply: 5)
  43. qrt-pcr and northern blot ,which way is better? - (reply: 4)
  44. microrna quantitation ---use northern blot or qrt-pcr? - (reply: 1)
  45. confirming an siRNA gene knockdown using qRT-PCR - (reply: 1)
  46. Amplify cDNA for qRT-PCR? - (reply: 6)
  47. qRT-PCR no of cycles?? - (reply: 6)
  48. Is my primer going to work for qRT-PCR? - (reply: 6)
  49. Cell-cycle independant qRT-PCR internal control - anyone has experience doing this? (reply: 1)
  50. Ncode qRT-PCR - (reply: 6)
  51. qRT-PCR calculations - Averaging across biological replicates (reply: 2)
  52. Endogenous control in bacterial RT-qPCR - (reply: 4)
  53. qRT-PCR Problems - (reply: 1)
  54. qRT-PCR with DMSO - (reply: 2)
  55. Problems with qRT-PCR - (reply: 3)
  56. Optimizing multiplex RT-qPCR - (reply: 3)