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Lowry Protein Assay

Source: Protocol Online
Date Added: Tue May 14 2002
Date Modified: Tue Feb 17 2009

 reagents

  1. 2% Na2CO3 in 0.1 N NaOH
  2. 2% NaK Tartrate
  3. 1% CuSO45H2O
    Mixture before use:  25.00 ml of 1
                                  0.25 ml of 2
                                  0.25 ml of 3
  4.  2N Folin reagent (yellow in color)
  5.  Standard: Given BSA 1 mg/ml

 procedure

  1. Transfer sample containing up to 40 g of protein to test tubes and bring volume up to 200 L with H2O.
  2. Add 1 mL of mixture and mix well with the Vortex.
  3. Incubate for 10 minutes.at Room Temperature.
  4. Add 50 L of Folin reagent, mix well with Vortex.
  5. Incubate for 30 minutes at Room Temperature.
  6. Read at 540 nm against an H2O blank.
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