Protocols
- Lowry Protein Assay (Yu-li Wang's Lab, University of Massachusetts Medical School )
Detailed procedures with materials.
http://ylwang.umassmed.edu/protocol/pa/lowry.htm
Added: Fri Oct 17 2003, Hits: 2870, Reviews: 0 Write review Cached - Lowry Protein Determination (Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
The method is based on both the Biuret reaction, where the peptide bonds of proteins react with copper under alkaline conditions producing Cu+, which reacts with the Folin reagent, and the Folin-Ciocalteau reaction, which is poorly understood but in essence phosphomolybdotungstate is reduced to heteropolymolybdenum blue by the copper-catalyzed oxidation of aromatic amino acids. The reactions result in a strong blue color, which depends partly on the tyrosine and tryptophan content.
http://wolfson.huji.ac.il/purification/Protocols/l...
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Lowry Protein Assay
(P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
The Lowry procedure is one of the most venerable and widely-used protein assays, being first described in 1951 [Lowry et al., J. Biol. Chem. 193: 265-275 (1951)]. Under alkaline conditions, copper complexes with protein. When folin phenol reagent (phospho-molybdic-phosphotungstic reagent) is added, the Folin-phenol reagent binds to the protein. Bound reagent is slowly reduced and changes color from yellow to blue.
http://www.animal.ufl.edu/hansen/protocols/lowry.h...
Added: Tue May 14 2002, Hits: 4802, Reviews: 0 Write review Cached - Lowry Protein Assay ( Protocol Online)
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Lowry Protein Assay
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
http://homepages.gac.edu/~cellab/appds/appd-g.html...
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Lowry-Peterson Protein Determination
(Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
Protein determination according to Lowry procedures for protein solution with interference, or for membrane extracts.
http://wolfson.huji.ac.il/purification/Protocols/l...
Added: Tue Feb 17 2009, Hits: 2006, Reviews: 0 Write review Cached -
Modified Lowry Protein Assay
(Hancock Lab)
The advantage of this assay over the Lowry assay from which it is derived, is the presence of SDS in reagent A. This allows one to perform protein assays in nearly any detergent (eg., when detergents are used in the purification of outer membrane proteins). In addition, SDS separates membrane proteins from contaminating membrane constituents and denatures the proteins allowing more reproducible results.
http://www.cmdr.ubc.ca/bobh/methods/PROTEINASSAY.h...
Added: Tue May 14 2002, Hits: 3207, Reviews: 0 Write review Cached