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Ambion's Tips From the Bench: RNA Conversions
Tips from the Bench

RNA Conversions

When isolating RNA, it helps to have a rough idea of how much total or poly(A) RNA can be recovered from a given amount of tissue or cells. It is also beneficial to know rough expression levels for rare to moderately abundant transcripts when performing Northern analysis or RPAs, e.g. in order to set up a meaningful standard curve for absolute quantitation, or to know how long of an exposure is reasonable. These numbers provide a point of reference against which experimental procedures and results can be checked. Tables 1 and 2 provide general guidelines for estimating RNA yields from a variety of cells and tissues. Remember that RNA yields from analogous tissues in different organisms can also vary:

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Ordering Information
For prices and availability, please contact Customer Service.

Cat# Product Name Size
1356 RT-PCR Competitor Construction Kit 10 rxns
1710 RETROscript® Kit 40 rxns
1716 QuantumRNA™ Classic 18S Internal Standard 100 rxns
1717 QuantumRNA™ Classic II 18S Internal Standard 100 rxns
1718 QuantumRNA™ Universal 18S Internal Standard 100 rxns
1720 QuantumRNA™ Beta-actin Internal Standards 100 rxns
1902 Paraffin Block RNA Isolation Kit 100 rxns
1910 ToTALLY RNA™ Kit 10 g tissue
1911 RNAqueous®-Midi Kit 15 purifications
1912 RNAqueous® Kit 50 purifications
1913 RNAqueous®-Blood Module 50 purifications
1914 RNAqueous®-4PCR Kit 30 rxns
1915 Poly(A)Pure™ Kit 6 rxns
1918 MicroPoly(A)Pure™ Kit 20 rxns
1920 RNAqueous®-96 Kit 192 purifications
2050 SuperTaq™ Polymerase (Cloned) 5 U/µl 50 U
2052 SuperTaq™ Polymerase (Cloned) 5 U/µl 250 U
2054 SuperTaq™ Plus Polymerase (Cloned) 5 U/µl 50 U
2056 SuperTaq™ Plus Polymerase (Cloned) 5 U/µl 250 U
9690 Plant RNA Isolation Aid 10 ml
9736 RNAwiz™ 100 ml
9890 DNAZap 250 ml of each solution
10035 96-well PCR Plate w/ 8 Strip Caps 10 plates
10036G 96-well PCR Plate Replacement Caps 125 strips
1906 DNA-free 50 rxns
10065 Spin Columns and Tubes 100 units
12225 Thin-walled, Frosted Lid, RNase-free PCR Tubes 1000 x 0.2 ml
12230 8-strip PCR Tubes & Caps, 0.2 ml 125 strips (1000 tubes)
12250 Thin-walled, Dome Cap, RNase-free PCR Tubes 1000 x 0.5 ml
12275 Thin-walled, Frosted Lid, RNase-free PCR Tubes 1000 x 0.5 ml
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