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Protocols
- In vitro RNA Synthesis (Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
In vitro RNA synthesis from plasmid-borne sequences under the control of T phage promoters.
http://www.nottingham.ac.uk/~mbzspd/methods/In_vit...
Added: Sat Apr 10 2004, Hits: 3310, Reviews: 0
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In Vitro RNA Transcription
(Promega)
For in vitro preparation single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts.
http://www.promega.com/tbs/TM016/Tm016.html#iv
Added: Tue May 14 2002, Hits: 5817, Reviews: 0
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in vitro Transcription
(Brent Graveley)
http://penguin.uchc.edu/~intron/Protocols/in%20vit...
Added: Tue May 14 2002, Hits: 3994, Reviews: 0
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In Vitro Transcription Assay (Run-off Assay) using Permeabilized Cells
(BioProtocol)
With this protocol, transcripts that were initiated from specific genes by RNA polymerases prior to permeabilization can be measured. Instead of a nuclear extract, permeabilized cells are used.
http://www.bioprotocol.com/protocolstools/protocol...
Added: Thu Oct 03 2002, Hits: 2242, Reviews: 0
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In Vitro Transcription of RNA
(The Science Advisory Board)
This protocol produces RNA transcripts from a cloned gene that can then be used in an in vitro translation assay.
http://www.scienceboard.net/resources/protocols.as...
Added: Tue Apr 26 2005, Hits: 2781, Reviews: 0
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In vitro Transcription Reaction
(IVAN J. DELGADO ORLIC)
http://www.msu.edu/~delgadoi/140.html
Added: Tue May 14 2002, Hits: 2715, Reviews: 0
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In Vitro Transcription with SP6 Polymerase
(The Science Advisory Board)
http://www.scienceboard.net/resources/protocols.as...
Added: Sun Jun 05 2005, Hits: 853, Reviews: 0
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In Vitro Transcription with T7 RNA Polymerase
(Sauer Lab, MIT)
http://openwetware.org/wiki/Sauer:In_vitro_transcr...
Added: Thu Mar 08 2007, Hits: 1315, Reviews: 0
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Pol II in vitro Transcription and Primer Extension Assay
(The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
http://www.fhcrc.org/labs/hahn/methods/biochem_met...
Added: Tue May 14 2002, Hits: 685, Reviews: 0
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Pol III in vitro Transcription
(Hahn Lab)
http://www.fhcrc.org/labs/hahn/methods/biochem_met...
Added: Tue May 14 2002, Hits: 422, Reviews: 0
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