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Protocols
- Thrombin Cleavage of a GST fusion protein (Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
In many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix. The amount of thrombin, temperature and length of incubation must be calibrated for each system.
http://wolfson.huji.ac.il/purification/TagProteinP...
Added: Sat Feb 21 2009, Hits: 503, Reviews: 0 Write review Cached -
Cleavage Sites Table for Fusion Proteins
http://wolfson.huji.ac.il/purification/TagProteinP...
Added: Sat Feb 21 2009, Hits: 204, Reviews: 0 Write review Cached -
Factor Xa Cleavage of MBP-Fusion protein
(Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
http://wolfson.huji.ac.il/purification/TagProteinP...
Added: Sat Feb 21 2009, Hits: 142, Reviews: 0 Write review Cached