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Small scale His-Tag purification under nature conditions The Protein Purification Facility
The Wolfson Centre for Applied Structural Biology
The Hebrew University of Jerusalem
Dr. Mario Lebendiker
mariol@mail.ls.huji.ac.il  Tel: 972-2-6586920 

Cleavage Sites Table for Fusion Proteins

PROTEASE
 COMPANY
 Protease Capture
Thrombin
Leu-Val-Pro-Arg▼Gly-Ser
 Amersham-Biosciences, Novagen,
 SIGMA, Roche
 Benzamidine-Agarose
Factor Xa
Ile-Glu/Asp-Gly-Arg▼ 
 Amersham-Biosciences, New England Biolabs,
Roche
 Benzamidine-Agarose
Enterokinase
Asp-Asp-Asp-Asp-Lys▼
 New England Biolabs,
 Novagen, Roche
 Trypsin Inhibitor-Agarose
TEV protease
Glu-Asn-Leu-Tyr-Phe-Gln▼Gly
 Invitrogen – Life Technologies
  Ni-NTA (6His recomb. TEV)
PreScission
Leu-Glu-Val-Leu-Phe-Gln▼Gly-Pro
 Amersham-Biosciences
 GSTrap for GST fusion enzyme
TAGZyme
His-tag removal by Exoproteolytic Digestion
 Quiagen
 Ni-NTA (6His recomb. enzyme)
Intein Site
dithiothreitol cleavage
 New England Biolabs
 DTT elimination by dialysis 
HRV 3C Protease 
Leu-Glu-Val-Leu-Phe-Gln▼Gly-Pro
 Novagen   
Ni-NTA (6His recomb. enzyme)
SUMO Protease
recognize the tertiary structure of the ubiquitin-like (UBL) protein, SUMO
Invitrogen – Life Technologies, LifeSensors
 Ni-NTA (6His recomb. enzyme)

 
 
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Dr. Mario Lebendiker The Protein Purification Facility
The Wolfson Centre for Applied Structural Biology,    The Hebrew University of Jerusalem
mariol@mail.ls.huji.ac.il  Tel: 972-2-6586920 

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