Top : Molecular Biology : PCR : Other PCR Methods

Subcategories

Protocols
  • 384-well PCR (Practical Molecular Biology)
    For bacteria clone screening.
    http://molbiol.ru/eng/protocol/21_04.html
    Added: Sat Oct 12 2002, Hits: 896, Reviews: 0  Write review   Cached
  • Amplification of Genomic DNA using Alu PCR PDF file (Thomas Ried's Lab, NCI)
    http://www.riedlab.nci.nih.gov/publications/2436.1...
    Added: Fri Oct 18 2002, Hits: 1250, Reviews: 0  Write review  
  • Asymmetric PCR for ssDNA Production (Molecular Biology Techniques Manual)
    http://web.uct.ac.za/microbiology/pcrcond.htm#Asym...
    Added: Tue May 14 2002, Hits: 1220, Reviews: 0  Write review   Cached
  • Core sample PCR (NWFSC)
    A method to re-PCR unique bands from products of mixed size
    http://micro.nwfsc.noaa.gov/protocols/core_sample_...
    Added: Tue May 14 2002, Hits: 808, Reviews: 0  Write review  
  • Detection of Viruses in Infected Plant Extracts using Immunocapture-PCR (Protocol Online)
    Diagnostic PCR should fulfill several qualitative characteristics, but the most important among them are specificity, sensitivity, efficiency and reproducibility of detection of viruses. Immunocapture-PCR (IC-PCR) may be used in detecting viruses using clarified plant extracts with degenerate primers, without prior isolation of total nucleic acids. It is especially useful for detecting viruses that exist in low or variable titers en planta and in plant species which contain various forms of PCR amplification inhibitors (polysaccharides, tannins, polyphenolics etc.).
    Added: Mon Feb 02 2009, Reviews: 0  Write review  
  • Direct PCR from Whole Yeast Cells (Namjin Chung)
    Zymolyase Method for screening yeast transformants
    http://www.musc.edu/bcmb/ceramide/protocols/0003.h...
    Added: Tue May 14 2002, Hits: 898, Reviews: 0  Write review   Cached
  • Gene Disruption by Fusion PCR (E. Beasley and D. Amberg)

    http://www.upstate.edu/biochem/amberg/protocols/fu...
    Added: Tue May 14 2002, Hits: 466, Reviews: 0  Write review   Cached
  • Hot Start PCR (Molecular Biology Techniques Manual)
    http://web.uct.ac.za/microbiology/pcrcond.htm#Hot
    Added: Tue May 14 2002, Hits: 1303, Reviews: 0  Write review   Cached
  • Incorporation of Digoxigenin-dUTP into Plasmid Inserts Using PCR PDF file (CIMMYT Applied Molecular Genetics Laboratory)
    http://www.cimmyt.org/english/docs/manual/protocol...
    Added: Mon Aug 05 2002, Hits: 876, Reviews: 0  Write review  
  • Non-organic Isolation of Tail DNA and PCR (Jackson Labs)
    Using tail tissue directly for PCR after proteinase K digestion.
    http://www.jax.org/imr/tail_nonorg.html
    Added: Tue May 14 2002, Hits: 402, Reviews: 0  Write review   Cached
  • PCR Amplification of Inserts from Bacterial Cultures PDF file (CIMMYT Applied Molecular Genetics Laboratory)
    PCR amplification of plasmid insert without plasmid preparation
    http://www.cimmyt.org/english/docs/manual/protocol...
    Added: Mon Aug 05 2002, Hits: 3944, Reviews: 0  Write review  
  • PCR amplification of rRNA gene fragment. (Jeffrey D. Newman, Lycoming College)
    http://srv2.lycoming.edu/~newman/rrna/PCR.htm
    Added: Tue May 14 2002, Hits: 395, Reviews: 0  Write review   Cached
  • PCR for Radiation Hybrid Mapping (Stanford Human Genome Center)
    A protocol used to assay for the presence of an STS for radiation hybrid mapping
    http://shgc-www.stanford.edu/Mapping/rh/procedure/...
    Added: Tue May 14 2002, Hits: 349, Reviews: 0  Write review   Cached
  • PCR from Plant Tissue (Schneitz Lab, University of Zurich)
    Use plant such as leaves or flowers tissue directly for PCR without DNA extraction
    http://www.unizh.ch/botinst/Cyto_Website/schneitzL...
    Added: Tue May 14 2002, Hits: 2804, Reviews: 0  Write review   Cached
  • PCR Manual (BMB)
    This manual contains detailed protocols on performing PCR as well as preparation of templates and post-PCR clean-up. Important applications such as PRINS, cycle sequencing, detection of human chromosomes, and non-radioactive labeling are discussed.
    http://206.53.227.20/prod_inf/manuals/pcr_man/pcr_...
    Added: Tue May 14 2002, Hits: 443, Reviews: 0  Write review   Cached
  • PCR of Blood, Hair or Small Tissue Samples (Gary A. J. Brown)
    Amplify DNA from a drop of Blood.
    http://ourworld.compuserve.com/homepages/TheBroons...
    Added: Tue May 14 2002, Hits: 491, Reviews: 0  Write review   Cached
  • Preparation of Alpha-satellite DNA by PCR PDF file (Thomas Ried's Lab, NCI)
    Detailed procedures including Primary PCR for amplification of alpha satellite sequences, Secondary PCR for labeling of alpha satellite sequences, FISH hybridizations of alpha satellite DNA probes.
    http://www.riedlab.nci.nih.gov/publications/2443.3...
    Added: Fri Oct 18 2002, Hits: 751, Reviews: 0  Write review  
  • Primary Amplification of Genomic DNA using DOP - PCR PDF file (Thomas Ried's Lab, NCI)
    http://www.riedlab.nci.nih.gov/publications/2435.3...
    Added: Fri Oct 18 2002, Hits: 1002, Reviews: 0  Write review  
  • Single Primer (Semi-Random) PCR (John Roth, Division of Biological Sciences,University of California at Davis)
    Single primer PCR allows amplification from known to unknown regions in chromosomes, phage, plasmids, large PCR products and other sources of DNA.
    http://rothlab.ucdavis.edu/protocols/semirandom.ht...
    Added: Tue May 25 2004, Hits: 859, Reviews: 0  Write review   Cached
  • Single Tube Confirmation PCR Protocol (Saccharomyces Genome Deletion Project)
    A colony PCR strategy is used to identify successful deletions. This method is fast, reliable, and amenable to a 96-well format
    http://sequence-www.stanford.edu/group/yeast_delet...
    Added: Tue May 14 2002, Hits: 875, Reviews: 0  Write review   Cached
  • Site-directed Mutagenesis using PCR (NWFSC)
    Use of PCR in site-directed mutagenesis accomplishes strand separation by using a denaturing step to separate the complementing strands and allowing efficient polymerization of the PCR primers. PCR site-directed methods thus allow site-specific mutations to be incorporated in virtually any double-stranded plasmid; eliminating the need for M13-based vectors or single-stranded rescue.
    http://micro.nwfsc.noaa.gov/protocols/pcr-mut.html
    Added: Tue May 14 2002, Hits: 2117, Reviews: 0  Write review  
  • Standard PCR Protocol (Molecular Biology Techniques Manual)
    The followings are described in detail
    Recommended Reagent Concentrations
    Recommended Reaction Conditions
    "Hot Start" PCR
    Asymmetric PCR for ssDNA Production
    Detecting Products
    Labeling PCR Products with Digoxigenin
    Cleaning PCR Products
    Sequencing PCR Products
    Cloning PCR Products
    http://web.uct.ac.za/microbiology/pcrcond.htm#Labe...
    Added: Tue May 14 2002, Hits: 4268, Reviews: 0  Write review   Cached