Visualize oligos by UV shadowing or methylene blue staining
Added: Tue May 14 2002, Hits: 636, Reviews: 0
UV shadowing is a technique for visualizing nucleic acids, e.g. separated in a gel, using short-wave UV light (254 nm) and a fluor-coated TLC plate. This method is for identifying nonisotopic probes or unlabeled RNA (e.g. for microinjection) during gel purification since the other alternative of staining with EtBr or acridine orange requires subsequent extraction of the dye. However, this method can be used to visualize any nucleic acid present in sufficient amount (> 0.3 µg).
Added: Tue May 14 2002, Hits: 467, Reviews: 0