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Protocols
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Fork-Direction 2-D Gels
(Fangman/Brewer)
For detecting direction of replication fork movement
http://fangman-brewer.genetics.washington.edu/fork...
Added: Tue May 14 2002, Hits: 1074, Reviews: 0 Write review Cached -
RLGS protocol
(Makoto Kawase)
Detailed Restriction Landmark Genomic Scanning (RLGS) protocol. Although this protocol is for plant genome, it can also be applied to other genome. This technique has been used for genome-wide methylation analysis and has the name of Restriction Landmark Genomic Scanning for Methylation (RLGS-M).
http://www.geocities.jp/millet_kawase/RLGS/protoco...
Added: Tue May 14 2002, Hits: 1981, Reviews: 0 Write review -
Standard 2-D Gel of DNA Replication Intermediatess
(Fangman/Brewer)
The first dimension gel is intentionally run at low voltage in low percentage agarose to separate DNA molecules in proportion to their mass. The second dimension is run at high voltage in a gel of higher agarose concentration in the presence of Ethidium bromide so that the mobility of a non-linear molecule is drastically influenced by its shape.
http://fangman-brewer.genetics.washington.edu/2Dge...
Added: Tue May 14 2002, Hits: 1110, Reviews: 0 Write review Cached