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Protocols
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Southern Transfer to Nylon Membranes
(Donis Keller Lab)
Southern transfer is the classic method used to move DNA (usually in the form of restriction fragments) from agarose gels to a solid support, e. g. nylon membrane. This protocol is designed for transferring DNA for RFLP analysis. The optimum size range of DNA fragments detected this way is 2 - 25 kb.
http://humgen.wustl.edu/hdk_lab_manual/southern/so...
Added: Tue May 14 2002, Hits: 980, Reviews: 0 Write review Cached -
Standard Hybridization Technique for DNA
(Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
The standard solution typically used for both pre-hybridisation and hybridisation is based on that given in Maniatis et al., (1982) with both Denhart's solution and heterologous DNA being replaced by heparin (Singh and Jones, 1984).
http://www.nottingham.ac.uk/~mbzspd/methods/Standa...
Added: Sat Apr 10 2004, Hits: 148, Reviews: 0 Write review Cached -
Standard Southern Blot Procedure
(Penn Cardiovascular Institute)
Procedures include DNA digestion, solutions, prehybridization reaction, [32P]-labelling of probe, hybridization and stripping southern blot on nitrocellulose.
http://www.med.upenn.edu/mcrc/parmacek_lab/Souther...
Added: Sun Nov 01 2009, Hits: 716, Reviews: 0 Write review Cached -
Worm genomic Southern blots
(Laboratory of Michael R. Koelle, Yale University)
Includes DNA preparation, hybridization, and membrane stripping procedures.
http://www.med.yale.edu/mbb/koelle/protocols/proto...
Added: Sun Nov 01 2009, Hits: 497, Reviews: 0 Write review Cached