Making Combination Markers for Size Standards on Southern Blots
(Donis Keller Lab)
Digesting lambda DNA (BRL) with a specific restriction enzyme will result in lambda DNA fragments of known size. Three separate lambda digests (BglII, BstEII, and XhoI) will give 23 fragments with a molecular weight range of 60 bp to 33,498 bp (see illustration below). The three digests are pooled, diluted, and used as molecular weight size standards for Southern transfers.
Added: Tue May 14 2002, Hits: 1081, Reviews: 0