Protocols
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Labeling DNA with 32P by Primer Extension
(Donis Keller Lab)
Double stranded DNA is heat-denatured and random hexanucleotide primers are attached to the single strand. The synthesis is carried out using Klenow fragment of DNA polymerase I incorporating 32P-dCTP at the same time.
http://humgen.wustl.edu/hdk_lab_manual/southern/so...
Added: Tue May 14 2002, Hits: 345, Reviews: 0 Write review Cached -
Preparation of Radioactive Probes for Southern Blotting
(Gottschling Lab,Fred Hutchinson Cancer Research Center)
PCR Method to Make Radioactive Probes
http://labs.fhcrc.org/gottschling/General%20Protoc...
Added: Sun Nov 01 2009, Hits: 35, Reviews: 0 Write review Cached -
Primer Extension Labeling of DNA in Low Melt Agarose
(Donis Keller Lab)
This procedure allows a restriction fragment(s) to be labeled directly from a gel slice, without the associated losses of DNA usually seen with gel purification methods.
http://humgen.wustl.edu/hdk_lab_manual/southern/so...
Added: Tue May 14 2002, Hits: 117, Reviews: 0 Write review Cached -
Probe DNA Preparation for Southern Blotting
(Whitelabs.org)
http://www.whitelabs.org/Lab%20Protocols/southern%...
Added: Sun Nov 01 2009, Hits: 47, Reviews: 0 Write review Cached