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Protocols

• Labeling DNA with 32P by Primer Extension (Donis Keller Lab)
  Double stranded DNA is heat-denatured and random hexanucleotide primers are attached to the single strand. The synthesis is carried out using Klenow fragment of DNA polymerase I incorporating 32P-dCTP at the same time.
  http://humgen.wustl.edu/hdk_lab_manual/southern/so...
  Added: Tue May 14 2002, Hits: 1114, Reviews: 0  Write review   Cached
• Preparation of Radioactive Probes for Southern Blotting (Gottschling Lab,Fred Hutchinson Cancer Research Center)
  PCR Method to Make Radioactive Probes
  http://labs.fhcrc.org/gottschling/General%20Protoc...
  Added: Sun Nov 01 2009, Hits: 955, Reviews: 0  Write review   Cached
• Primer Extension Labeling of DNA in Low Melt Agarose (Donis Keller Lab)
  This procedure allows a restriction fragment(s) to be labeled directly from a gel slice, without the associated losses of DNA usually seen with gel purification methods.
  http://humgen.wustl.edu/hdk_lab_manual/southern/so...
  Added: Tue May 14 2002, Hits: 381, Reviews: 0  Write review   Cached
• Probe DNA Preparation for Southern Blotting (Whitelabs.org)
  http://www.whitelabs.org/Lab%20Protocols/southern%...
  Added: Sun Nov 01 2009, Hits: 1354, Reviews: 0  Write review   Cached