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Protocols
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Oligo Purification on Acrylamide Gels
(Botwell Lab)
Purification of labeled oligos
http://grimwade.biochem.unimelb.edu.au/bowtell/mol...
Added: Tue May 14 2002, Hits: 117, Reviews: 0
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Oligo Purification on Acrylamide Gels
(Botwell Lab)
Purification of labeled oligos
http://grimwade.biochem.unimelb.edu.au/bowtell/mol...
Added: Tue May 14 2002, Hits: 82, Reviews: 0
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Oligonucleotide Purification by PAGE and DEAE
(The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
http://labs.fhcrc.org/hahn/Methods/mol_bio_meth/ol...
Added: Tue May 14 2002, Hits: 639, Reviews: 0
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Removal of Unincorporated dNTPs by Spin Columns
(CIMMYT Applied Molecular Genetics Laboratory)
This is an optional step after labeling by Nick Translation or Random Priming.
http://www.cimmyt.org/english/docs/manual/protocol...
Added: Mon Aug 05 2002, Hits: 706, Reviews: 0
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Spin Column chromatography
(Schimenti Lab)
Used to removed unincorporated nucleotides from labeling reactions.
http://lena.jax.org/~jcs/techniques/protocols/Spin...
Added: Tue May 14 2002, Hits: 77, Reviews: 0
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