This is a cached page for the URL (http://axon.med.harvard.edu/~cepko/protocol/mike/A2.html). To see the most recent version of this page, please click here.
Protocol Online is not affiliated with the authors of this page nor responsible for its content.
About Cache
Protocol.template

Protocol A.2

 

Antigen Retrieval

 

Some other useful protocols can be found in the following references:

Caltuvetti et. al., 1993, J. Pathology 171:83 and Shi et. al., 1991 J. Hist. Cytochem. 39:741.

Solutions

Denaturing Solution

0.8M urea

store at room temperature

 

Procedure

• Place the slides in a plastic coplin jar filled with denaturing solution and microwave on high for 5 minutes.

• Change the denaturing solution and repeat, cool to room temperature.

• Wash 5 times in PBS and proceed with staining as usual.