Overview: Immunocytochemistry is a technique used to detect specific antigens (protein) in live or fixed cell cultures by use of a specific antibody which recognize the protein in the cell.
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Protocols
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Embedding in Resins for Immunocytochemistry
(House Ear Institute)
Describes the use of the specialized and not so specialized resins.
http://www.hei.org/research/aemi/resin.htm
Added: Tue May 14 2002, Hits: 1535, Reviews: 0 Write review Cached -
Freeze Substitution for Embedding in Resins
(House Ear Institute)
A simple, inexpensive way of embedding in resin using freeze substitution methods.
http://www.hei.org/research/aemi/pmfs.htm
Added: Tue May 14 2002, Hits: 664, Reviews: 0 Write review Cached -
Immunocytocheimical Identidication of Proliferating Cells in Vascular Tissue (anti-BrdU); Diaminoben
(Wilcox Lab)
http://www.emory.edu/WILCOX/brduDAB.html
Added: Tue May 14 2002, Hits: 1347, Reviews: 0 Write review Cached -
Immunocytochemical Identification of Proliferating Cells in Vascular Tissue (anti-BrdU); Vector Red
(Wilcox Lab)
http://www.emory.edu/WILCOX/brdured.html
Added: Tue May 14 2002, Hits: 947, Reviews: 0 Write review Cached -
Immunocytochemistry Protocols
(Lamond Lab, University of Dundee)
In this section of Lamond Lab's collection of protocols, you can find the following protocols:
- Conjugating antibodies
- In vivo transcription
- Paraspeckle staining
- Cell fix - PFA
- Cell fix - MeOH
- Immunostaining (full protocol)
- Preparing DNA/protein for microinjection
- BrUTP labeling
http://www.lamondlab.com/LTprotImmCyt.htm
Added: Thu May 08 2003, Hits: 6819, Reviews: 0 Write review Cached -
Immunocytochemistry with Adherent Cells
(Upstate)
Standard protocol for immunocytochemistry by growing cell on microscopic slides, fixing and immunostaining with antibody.
http://www.millipore.com/techpublications/tech1/mc...
Added: Tue Jul 27 2004, Hits: 6391, Reviews: 0 Write review Cached -
Immunocytochemisty-Fluorescence Protocol
(R&D)
General protocol including details on staining cell in suspension and on slide
http://www.rndsystems.com/ihc_detail_objectname_Fl...
Added: Tue May 14 2002, Hits: 5171, Reviews: 0 Write review Cached -
Immunocytohistochemistry
(Howell Lab)
Procedures for cell plating, fixing, antibody incubation and mounting
http://cancer.ucsd.edu/howelllab/Immunocyto.html
Added: Tue May 14 2002, Hits: 6216, Reviews: 0 Write review Cached -
Immunohisto- and Immunocyto-Chemisty-Enzymatic Protocol
(R&D)
General and nice protocol for immunocyto and immunohistochemistry including sample preparation, staining and trouble shooting
http://www.rndsystems.com/ihc_detail_objectname_En...
Added: Tue May 14 2002, Hits: 2919, Reviews: 0 Write review Cached -
Immunostaining (Immunohistochemistry and Immunocytochemistry) Protocols
(Millpore)
Protocols for Cryostat Sections, Paraffin-embedded Sections, and General Protocol for Immunohistochemical Staining, Heat Induced Epitope Retrieval (HIER) Technique for Paraffin Sections and Immunocytochemistry General Protocol
http://www.millipore.com/immunodetection/id3/immun...
Added: Wed Feb 13 2008, Hits: 1526, Reviews: 0 Write review Cached