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Protocols: Immunocytochemistry

**Protocols are downloaded as PDF files which can be opened with the free Adobe Acrobat Reader

Conjugating antibodies to fluorescent probes

In vivo transcription

Staining paraspeckles

Cell fixation - paraformaldehyde

Cell fixation - methanol

Fixation, permeabilization and immunostaining of cells

Preparing proteins/antibodies/DNA/RNA for microinjection

BrUTP labeling of RNA transcripts

NB: These protocols are intended for use by trained personnel only, in a research laboratory environment.

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Last Updated April 22, 2003