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Protocols
  • A Simplified System for Rapid Generation of Recombinant Adenoviruses ( )
    Give detailed guide to the construction of recombinant adenoviruses.
    http://coloncancer.org/adeasy.htm
    Added: Tue May 14 2002, Hits: 233, Reviews: 0  Write review   Cached
  • Coating complexes with pHPMA (Gene Delivery Group, University of Birmingham)
    http://web.bham.ac.uk/can4psd4/brum/coating.html
    Added: Wed Sep 04 2002, Hits: 58, Reviews: 0  Write review   Cached
  • Ethidium Bromide assay for DNA complex formation (Gene Delivery Group, University of Birmingham)
    http://web.bham.ac.uk/can4psd4/brum/etbr.html
    Added: Wed Sep 04 2002, Hits: 88, Reviews: 0  Write review   Cached
  • Gel Retardation assay for complex formation (Gene Delivery Group, University of Birmingham)
    The condensation of DNA by cationic polymers can be measured by a reduction of the mobility of DNA on agarose that leads to the retention of DNA in the wells
    http://web.bham.ac.uk/can4psd4/brum/agargel.html
    Added: Wed Sep 04 2002, Hits: 104, Reviews: 0  Write review   Cached
  • Isolation of complexes from serum (Gene Delivery Group, University of Birmingham)
    Complexes can be isolated from serum by centrifugation on either a sucrose or Percoll gradient. The sucrose gradient is the easier of the two to set up
    http://web.bham.ac.uk/can4psd4/brum/serum.html
    Added: Wed Sep 04 2002, Hits: 156, Reviews: 0  Write review   Cached
  • Nulcease Degradation Assay (Gene Delivery Group, University of Birmingham)
    http://web.bham.ac.uk/can4psd4/brum/nuclease.html
    Added: Wed Sep 04 2002, Hits: 73, Reviews: 0  Write review   Cached
  • Surgical organ perfusion method for somatic gene transfer (Teija Parpala-Spårman, Oulu University)
    A closed-circuit organ perfusion method for in vivo gene transfer. This method led to effective, up to 75% gene transfer into the renal glomeruli as assessed after four days. In the spleen, the perfusion method resulted in relatively effective gene transfer into perifollicular splenic cells, mostly macrophages and endothelial cells. Lung perfusion yielded transgene expression in alveolar epithelial cells, bronchiolar epithelial cells and, to a lesser extent, arteriolar endothelial cells and alveolar macrophages. Perfusion of the goats mammary gland using a retroviral vector in three experiments resulted in growth hormone secretion into the milk.
    http://herkules.oulu.fi/isbn9514255429/html/
    Added: Fri Aug 30 2002, Hits: 364, Reviews: 0  Write review   Cached