Isolation of complexes from serum
Complexes can be isolated from serum by centrifugation on either a sucrose or Percoll gradient. The sucrose gradient is the easier of the two to set up.
Isolating complexes from a Percoll gradient requires that an optimal gradient be determined for each complex to be isolated. For example, pLL/DNA complexes (pLL Mw 24,000) centrifuge into a Percoll layer of 1.04 g/ml. The best separation of serum from complexes was found using a gradient consisting of 2 mls of water, 3 mls 1.01 g/ml Percoll, 3 mls 1.02 g/ml, 2 mls 1.03 g/ml, 1 ml 1.04 g/ml, 1 ml 1.06 g/ml and 1 ml of 1.1 g/ml. Other complexes required different gradients for optimal separation of complexes from serum.
|Non-Viral Vectors||Viral Vectors|
|Polymer Vectors||European Collaboration|
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