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Protocols
  • cDNA/AFLP (CWB Bachem)
    The protocol which follows gives a step-by-step description of cDNA-AFLP, a method for visualisation of differential gene expression
    http://www.spg.wau.nl/pv/staff/aflp.htm
    Added: Tue May 14 2002, Hits: 779, Reviews: 0  Write review   Cached
  • Differential Display (Dr. Chun-Ming Liu, Plant Research International)
    The following procedures are described:
    RNA extraction and qualification DNase treatment of RNA sample
    Reverse transcription
    PCR amplification
    Separation on acrylamide gels
    DNA extraction from bands of interest
    Re-amplification by PCR
    Separation on agarose gels
    Excision of amplified products
    http://www.hybtech.org/Liu/DISPLAY.html
    Added: Tue May 14 2002, Hits: 4211, Reviews: 0  Write review   Cached
  • Differential Display (Breeden Lab)
    Detailed protocol for differential display
    http://www.fhcrc.org/labs/breeden/Methods/differen...
    Added: Tue May 14 2002, Hits: 1911, Reviews: 0  Write review   Cached
  • Differential Display PDF file (Clontech)
    Provides detailed guide to differential display technique
    http://www.clontech.com/techinfo/manuals/PDF/PT117...
    Added: Tue May 14 2002, Hits: 3546, Reviews: 0  Write review  
  • Differential Display-Reverse Transcription-PCR (Gerard Lazo)
    http://wheat.pw.usda.gov/~lazo/methods/lazo/ddrtpc...
    Added: Tue May 14 2002, Hits: 1753, Reviews: 0  Write review   Cached
  • Serial Analysis of Gene Expression (SAGE)
    SAGE is a powerful tool that allows the analysis of overall gene expression patterns with digital analysis. Because SAGE does not require a preexisting clone, it can be used to identify and quantitate new gene as well as know genes.
    http://www.sagenet.org/
    Added: Tue May 14 2002, Hits: 1245, Reviews: 0  Write review   Cached
  • Targeted Differential Display (Molecular Profiling Initiative, NCI)
    Protocol for differential gene expression analysis of cells obtained from microdissected tissue.
    http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAn...
    Added: Tue May 14 2002, Hits: 1097, Reviews: 0  Write review   Cached
  • What's Differential Display (GenHunter)
    Introduction to differential display technique
    http://www.genhunter.com/products/differential/
    Added: Tue May 14 2002, Hits: 2650, Reviews: 0  Write review   Cached