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Breeden Lab, differential dispaly

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Differential Display of RNAs

The differential display protocol described here is based on the principle described by:
Liang, P. and A.B. Pardee : Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction, Science 257, 967-971 (1992)

The oligonucleotides we use are kits, contaning 20 decamers, from
Operon Technologies Inc..

 I. DNase I treatment of RNA

 II. cDNA synthesis

 III. PCR reaction

 IV. denaturing gel

 V. isolation of PCR fragment

 VI. reamplification