This is a cached page for the URL ( To see the most recent version of this page, please click here.
Protocol Online is not affiliated with the authors of this page nor responsible for its content.
About Cache
Differential Display of Cotton Transcripts

Differential Display of Cotton Transcripts

Song, P., Yamamoto, E., and Allen, R. (1995) Plant Mol. Biol. Rep. 13:174-181

Plant Materials

Cotton ovules (Gossypium hirsutum cv. Coker 312) were collected 8, 15, and 20 days after anthesis. Total RNA was extracted from stripped ovules (fibers removed) and isolated fibers using the procedure of John (1992).

Reagents and Solutions


DNase I Treatment

First-strand DNA Synthesis

PCR Amplification

Non-denaturing Sequencing Gel Electrophoresis

Recovery, Reamplification, and Cloning of cDNA fragments