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Protocols

• Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells (Journal of Visualized Experiments)
  A video and text protocol for differentiating mouse ES cells including the following procedures: Assembling handing drops differentiation culture (EB formation), Collect undifferentiated ES cells, Assemble hanging drop culture, Collecting differentiated ES cells form hanging drops, Starting EB culture, EB dissociation and infection, Preparation of ES cell-derived progenitors for FACS and Transplantation.
  http://www.jove.com/index/Details.stp?ID=162
  Added: Wed Jan 28 2009, Hits: 1220, Reviews: 0  Write review   Cached
• Adipogenic Differentiation of Mesenchymal Stem Cells (R&D Systems)
  An easy to follow protocol describing a method to differentiate mesenchymal stem cells along the adipogenic lineage.
  http://www.rndsystems.com/stem_cell_protocol_detai...
  Added: Fri Mar 20 2009, Hits: 826, Reviews: 0  Write review   Cached
• Differentiate ES cells into cardiac myocytes (Nagy Lab, Mount Sinai Hospital )
  http://www.mshri.on.ca/nagy/Protocols/cardiac.htm
  Added: Wed Jan 28 2009, Hits: 937, Reviews: 0  Write review   Cached
• Differentiate ES cells into cystic embryoid bodies (Nagy Lab, Mount Sinai Hospital )
  http://www.mshri.on.ca/nagy/Protocols/cystemb.htm
  Added: Wed Jan 28 2009, Hits: 412, Reviews: 0  Write review   Cached
• Differentiate ES cells into glial cells and neurons (Nagy Lab, Mount Sinai Hospital )
  http://www.mshri.on.ca/nagy/Protocols/neurdif.htm
  Added: Wed Jan 28 2009, Hits: 882, Reviews: 0  Write review   Cached
• Directed differentiation of human embryonic stem cell to foregut endoderm (Beta Cell Biology Consortium)
  his protocol is for converting human embryonic stem cells to foregut endoderm in the presence of FGF4
  http://www.betacell.org/resources/data/protocols/v...
  Added: Sun Dec 20 2009, Hits: 316, Reviews: 0  Write review   Cached
• Directed differentiation of mouse embryonic stem cells to foregut endoderm (Beta Cell Biology Consortium)
  Optimized protocol for converting mouse embryonic stem cells to foregut endoderm in the presence of FGF4 and Wnt3a
  http://www.betacell.org/resources/data/protocols/v...
  Added: Sun Dec 20 2009, Hits: 238, Reviews: 0  Write review   Cached
• Directed differentiation of mouse embryonic stem cells to Sox17+ cells (Beta Cell Biology Consortium)
  This protocol is for converting mouse embryonic stem cells to Sox17+ cells using embryoid bodies in the presence of Activin A
  http://www.betacell.org/resources/data/protocols/v...
  Added: Sun Dec 20 2009, Hits: 245, Reviews: 0  Write review   Cached
• EB Differentiation (NIH Stem Cell Information)
  http://stemcells.nih.gov/research/nihresearch/scun...
  Added: Thu Jan 29 2009, Hits: 817, Reviews: 0  Write review   Cached
• ES Cell-derived Neuroepithelial Cell Cultures (Journal of Visualized Experiments)
  A common method to produce neural progenitors from ES cells is based on embryoid body (EB) formation, which reveals the differentiation of cells from all germ layers including neuroectoderm. An alternative and more efficient method to induce neuroepithelial cell development uses stromal cell-derived inducing activity (SDIA), which can be achieved by co-culturing ES cells with skull bone marrow-derived stromal cells (1). Both, EB formation and SDIA, reveal the development of rosette-like structures, which are thought to resemble neural tube- and/or neural crest-like progenitors. The neural precursors can be isolated, expanded and further differentiated into specific neurons and glia cells using defined culture conditions
  http://www.jove.com/index/details.stp?ID=118
  Added: Wed Jan 28 2009, Hits: 488, Reviews: 0  Write review   Cached
• Generate insulin-secreting islet clusters from human iPS (Beta Cell Biology Consortium)
  This protocol describes the method for generating insulin-producing islet clusters from human ES cell-like induced pluripotent stem cells derived from human foreskin fibroblast.
  http://www.betacell.org/resources/data/protocols/v...
  Added: Sun Dec 20 2009, Hits: 289, Reviews: 0  Write review   Cached
• In Vitro Differentiation of Embryonic Stem Cells (Rathmell Lab, University of North Carolina)
  http://www.unc.edu/~rathmell/protocols/DispaseES10...
  Added: Wed Jan 28 2009, Hits: 1038, Reviews: 0  Write review  
• In Vitro Differentiation of ES Cells into Cardiac Muscle (Nagy Lab)
  http://www.mshri.on.ca/nagy/Protocols/cardiac.htm
  Added: Tue May 14 2002, Hits: 732, Reviews: 0  Write review   Cached
• In Vitro Differentiation of ES Cells into Cystic Embryoid Bodies (Nagy Lab)
  http://www.mshri.on.ca/nagy/Protocols/cystemb.htm
  Added: Tue May 14 2002, Hits: 387, Reviews: 0  Write review   Cached
• In Vitro Differentiation of ES Cells into Neuronal Lineages (Nagy Lab)
  http://www.mshri.on.ca/nagy/Protocols/neurdif.htm
  Added: Tue May 14 2002, Hits: 823, Reviews: 0  Write review   Cached
• Mesenchymal Stem Cell Differentiation Protocols (Millipore)
  Osteogenesis differentiation from rat bone marrow-derived Mesenchymal stem cells.
  http://www.millipore.com/cellbiology/cb3/mesenchym...
  Added: Wed Jan 28 2009, Hits: 1327, Reviews: 0  Write review   Cached
• Osteogenic Differentiation of Mesenchymal Stem Cells (R&D Systems)
  A protocol to differentiate mesenchymal stem cells along the osteogenic lineage.
  http://www.rndsystems.com/stem_cell_protocol_detai...
  Added: Fri Mar 20 2009, Hits: 823, Reviews: 0  Write review   Cached