0:06 | Assembling handing drops differentiation culture |
0:08 | Collect undifferentiated ES cells |
2:24 | Prepare materials |
2:57 | Assemble hanging drop culture |
4:34 | Collecting differentiated ES cells form hanging drops |
6:05 | Starting EB culture |
6:53 | EB dissociation and infection |
11:25 | Preparation of ES cell-derived progenitors for FACS |
15:18 | Transplantation |
16:19 | Discussion |