Top : Cell Biology : Stem Cells : Differentiation of Stem Cell : Embryoid Body (EB) Formation
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Protocols
  • Embryoid Body Formation PDF file (ATCC)
    Embryoid Body (EB) culture is used to examine the differentiation potential of the embryonic stem (ES) cell line. The cells are grown using low-attachment dishes in the presence of complete growth medium lacking leukemia inhibitory factor (LIF). This process induces differentiation, permits suspension culture, and causes the cells to form aggregates. These aggregates are allowed to grow for several days or weeks and samples may be taken at various time points for analysis by flow cytometry or antibody staining. The following protocol was established for the formation of EBs from mouse embryonic stem cells and should not be used for human embryonic stem cells.
    http://www.atcc.org/Portals/1/Embryoid_Body_Format...
    Added: Wed Jan 28 2009, Hits: 3098, Reviews: 0  Write review  
  • Embryoid Body Formation Protocol PDF file (Invitrogen)
    http://www.invitrogen.com/etc/medialib/en/filelibr...
    Added: Wed Jan 28 2009, Hits: 2346, Reviews: 0  Write review  
  • In vitro Differentiation of Mouse Embryonic Stem (mES) Cells Using the Hanging Drop Method Video (Journal of Visualized Experiments)
    Hanging drop culture is a widely used EB formation induction method. The rounded bottom of hanging drop allows the aggregation of ES cells which can provide mES cells a good environment for forming EBs. The number of ES cells aggregatied in a hanging drop can be controlled by varying the number of cells in the initial cell suspension to be hung as a drop from the lid of Petri dish. Using this method we can reproducibly form homogeneous EBs from a predetermined number of ES cells.
    http://www.jove.com/index/details.stp?ID=825
    Added: Wed Jan 28 2009, Hits: 1708, Reviews: 0  Write review   Cached
  • Shrinky-Dink Hanging Drops: A Simple Way to Form and Culture Embryoid Bodies Video (Journal of Visualized Experiments)
    A simple, rapid, and scalable culture method to load pre-defined numbers of cells into microfabricated wells and maintain them for embryoid body development.
    http://www.jove.com/index/details.stp?ID=692
    Added: Wed Jan 28 2009, Hits: 961, Reviews: 0  Write review   Cached