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Protocols
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Ethanol-fixation of Samples for Long-term Storage and Subsequent DNA Staining
(The Janis V. Giorgi Flow Cytometry Laboratory, UCLA)
Procedure for cell fixation and staining.
http://cyto.mednet.ucla.edu/Protocols/ethanol.htm
Added: Sat Feb 11 2006, Hits: 3163, Reviews: 0
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Fixation and DNA Staining for Cell Cycle Analysis
(Iowa State University, Flow Cytometry Facility)
This method of DNA staining utilizes ethanol to fix the cells and permeabilize the membrane, which allows the dye (Propidium Iodide) to enter the cells.
http://www.biotech.iastate.edu/facilities/CELLHYB/...
Added: Tue May 14 2002, Hits: 7593, Reviews: 0
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Paraformaldehyde Fixation of Cells
(Iowa State University, Flow Cytometry Facility)
This fixation method is good for cells labeled by fluorochrome-conjugated antibodies to membrane antigens.
http://www.biotech.iastate.edu/facilities/CELLHYB/...
Added: Sat Mar 11 2006, Hits: 3276, Reviews: 0
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Preparation of 2% Formaldehyde Solution
(The Janis V. Giorgi Flow Cytometry Laboratory, UCLA)
Two methods of preparing 2% formaldehyde solution
http://cyto.mednet.ucla.edu/Protocols/preparat.htm
Added: Sat Feb 11 2006, Hits: 2173, Reviews: 0
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