This is a cached page for the URL ( To see the most recent version of this page, please click here.
Protocol Online is not affiliated with the authors of this page nor responsible for its content.
About Cache
Quick Paraformaldehyde Preparation


Mary-Ann Campbell's method.

This is a quick method of getting paraformaldehyde (PFM) into solution. It is a good alternative to the traditional methods which can take hours, and has the advantage that it is so quick that you are more inclined to prepare fresh PFM each time.

CAUTION! Flammable. Irritant vapor. Probably carcinogenic.


0.1 g paraformaldehyde powder in small glass tube
Add 0.5 ml distilled water
Add 1 drop 0.5 - 1.0 M sodium hydroxide

Heat to approx 80 °C for 2-3 mins; shake in water bath until PFM has dissolved. A beaker of very hot water will do.

Add 9.5 ml PBS. Correct pH with HCl if necessary.

Makes 10 ml of 1% PFM solution.

David Chambers: 960613; 960621
* Webmaster
* Salk Flow Cytometry home page