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Preparation of 2% Formaldehyde

 

PREPARATION OF 2% FORMALDEHYDE STOCK SOLUTION  (2 METHODS)

METHOD 1:

Formaldehyde preservative – 2% formaldehyde solution in protein-free phosphate-buffered saline (PBS).

Prepare as follows:

Add 2 g paraformaldehyde powder (e.g., Sigma, St. Louis, MO) to 100 ml of 1 X PBS. Heat to 70C (do not exceed this temperature) in a fume hood until the paraformaldehyde goes into solution (note that this happens quickly as soon as the suspension reaches 70C). Allow the solution to cool to room temperature. Adjust to pH 7.4 using 0.1 M NaOH or 0.1 M HCl, if needed. Filter and store at 4C protected from light.

 

METHOD 2:

Formaldehyde preservative – 2% formaldehyde solution in protein-free PBS.

Prepare as follows:

10% formaldehyde*

20 ml

10 x PBS

10 ml

Distilled water

70 ml

* 10% formaldehyde solution (e.g., Polysciences, Warrington, PA, ultrapure, Cat.#04018), depolymerized paraformaldehyde, EM grade, methanol-free solution.