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Protocols
- Preparation of Metaphase Chromosomes
(Thomas Ried's Lab, NCI)
http://www.riedlab.nci.nih.gov/publications/2413.3...
Added: Fri Oct 18 2002, Hits: 3545, Reviews: 0
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Amniotic Fluid Cultures on Coverslips
(Waisman Center Cytogenetics Lab, University of Wisconsin - Madison)
Amniotic fluid may be used for prenatal diagnosis of aneuploidy or other structural abnormalities.
http://www.slh.wisc.edu/cytogenetics/Protocols/Cul...
Added: Mon Dec 09 2002, Hits: 920, Reviews: 0
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Bone Marrow Culture for Chromosome Preparation
(Waisman Center Cytogenetics Lab, University of Wisconsin - Madison)
To identify chromosome anomalies in hematopoietic cells. Used especially for chromosome studies for hematological disorders such as preleukemia and leukemia.
http://www.slh.wisc.edu/cytogenetics/Protocols/Cul...
Added: Mon Dec 09 2002, Hits: 1327, Reviews: 0
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C-Banding
(Waisman Center Cytogenetics Lab, University of Wisconsin - Madison)
http://www.slh.wisc.edu/cytogenetics/Protocols/Sta...
Added: Mon Dec 09 2002, Hits: 525, Reviews: 0
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Centrosome Preparation
(Mitchison Lab)
http://mitchison.med.harvard.edu/protocols/mt1.htm...
Added: Tue May 14 2002, Hits: 1037, Reviews: 0
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CHO Chromosome Preparation
(Mitchison Lab)
http://mitchison.med.harvard.edu/protocols/chr1.ht...
Added: Tue May 14 2002, Hits: 1019, Reviews: 0
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Chorionic Villus Tissue for Chromosome Analysis
(Waisman Center Cytogenetics Lab, University of Wisconsin - Madison)
Chorionic villus tissue may be used for prenatal diagnosis of aneuploidy or other structural abnormalities. It is usually done between the 9th and 11th week of gestation. Chorionic villus is not derived from the actual fetus and in rare cases may not have the same karyotype as the fetus, giving false results.
http://www.slh.wisc.edu/cytogenetics/Protocols/Cul...
Added: Mon Dec 09 2002, Hits: 311, Reviews: 0
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Chromatin Electrophoresis
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
http://homepages.gac.edu/~cellab/chpts/chpt10/ex10...
Added: Tue May 14 2002, Hits: 747, Reviews: 0
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Chromosomal DNA Prep : cultured cells/tissue samples
(Mike A Dyer)
This protocol was developed for cultured cells but should be appropriate for dissociated tissues as well.
http://axon.med.harvard.edu/~cepko/protocol/mike/C...
Added: Tue May 14 2002, Hits: 743, Reviews: 0
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Distamycin A Plus DAPI Fluorescent Staining (Dst/DAPI)
(Waisman Center Cytogenetics Lab, University of Wisconsin - Madison)
In man, this technique produces brilliant fluorescence at the C-blocks of chromosomes 1, 9, and 16, at the heterochromatic part of the Y, and at a short arm segment of chromosome 15 (15pll).
http://www.slh.wisc.edu/cytogenetics/Protocols/Sta...
Added: Mon Dec 09 2002, Hits: 542, Reviews: 0
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