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Protocols
- Protocol for TSA Treatment of Mammlian Cells (The Epigenome Network of Excellence )
Provides background information and procedures how to optimize treatment conditions and to detect TSA effect on pericentric heterochromatin.
http://www.epigenome-noe.net/researchtools/protoco...
Added: Sat Mar 18 2006, Hits: 660, Reviews: 0 Write review Cached -
RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA direc
(Epigenome NoE)
new
Three protocols are explained in detail. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA, while the second protocol is a triple pulldown assay which essentially expands upon the dual pulldown to incorporate a third pulldown which is an iteration of the ChIP and is a pulldown for H3K27me3+. The third assay described here is the biotin-RNA pulldown of a low-copy RNA that spans the siRNA targeted promoter region
http://www.epigenome-noe.net/researchtools/protoco...
Added: Mon Aug 04 2008, Hits: 5, Reviews: 0 Write review -
TSA Treatment of Mammalian Cells
(Epigenome NoE)
new
Prolonged treatment of proliferating mammalian cells with low doses of a histone deacetylase inhibitor, trichostatin A, specifically affects pericentric heterochromatin. Relocation of these regions towards the nuclear periphery and loss of HP1 proteins ensue after several divisions. Meanwhile, DNA methylation state and core centromeric markers are maintained. Subsequent defects in centromeric function arise in mitosis. Remarkably, removal of the drug rapidly reverses all these changes
http://www.epigenome-noe.net/researchtools/protoco...
Added: Mon Aug 04 2008, Hits: 2, Reviews: 0 Write review -
Chromatin Preparation from Cultured Cells
(Chromatin and Gene Expression Group, The University of Birmingham, UK)
Detailed protocol with receipes.
http://medweb.bham.ac.uk/research/chromatin/protoc...
Added: Sun Jan 08 2006, Hits: 1337, Reviews: 0 Write review Cached