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Protocols
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IN-Gel Hybridization procedure for telomeric single stranded DNA detection
(Wellinger Lab, Université de Sherbrooke )
Telomeric DNA is replicated by a special mechanism in which an enzyme called telomerase will
elongate a short 3' overhang and create long, single stranded DNA at the ends of chromosomes. This enzyme requires a single stranded DNA portion as its substrate since the telomerase RNA moiety has to anneal with this DNA...
http://callisto.si.usherb.ca/~microbio/wellinger/p...
Added: Fri Jul 26 2002, Hits: 305, Reviews: 0
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Protein-DNA Telomere FISH
(Stewart Lab, Washington University at St. Louis)
http://www.stewartlab.wustl.edu/Protein_DNA_Telome...
Added: Thu May 27 2004, Hits: 424, Reviews: 0
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Telomeric Repeat Amplification Protocol (TRAP)
(Stewart Lab, Washington University at St. Louis)
A method for assessing telomerase activity
http://www.stewartlab.wustl.edu/trap_assay.htm
Added: Thu May 27 2004, Hits: 374, Reviews: 0
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Terminal Restriction Fragment (TRF) Southern Blotting
(Stewart Lab, Washington University at St. Louis)
To determine telomeric restriction fragment (TRF) length.
http://www.stewartlab.wustl.edu/TRF_southern_proto...
Added: Thu May 27 2004, Hits: 204, Reviews: 0
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