Overview: MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, first described by Mosmann in 1983, is based on the ability of a mitochondrial dehydrogenase enzyme from viable cells to cleave the tetrazolium rings of the pale yellow MTT and form a dark blue formazan crystals which is largely impermeable to cell membranes, thus resulting in its accumulation within healthy cells. Solubilisation of the cells by the addition of a detergent results in the liberation of the crystals which are solubilized . The number of surviving cells is directly proportional to the level of the formazan product created. The color can then be quantified using a simple colorimetric assay. The results can be read on a multiwell scanning spectrophotometer (ELISA reader).
Reference
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application toproliferation and cytotoxicity assays. J Immunol Methods. 1983 Dec 16;65(1-2):55-63.
Reference
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application toproliferation and cytotoxicity assays. J Immunol Methods. 1983 Dec 16;65(1-2):55-63.
Protocols
- Cell Proliferation assay (Shalini Jain and Hariom Yadav, Animal Biochemistry Division, National Dairy Research Institute, Karnal, Haryana, India)
This protocol will help to researchers working in cell biology, cancer biology etc with basic principle and introduction of method.
Added: Mon Aug 20 2007, Reviews: 0 Write review - MTT Assay (Lazo Lab, University of Pittsburgh)
Cells are treated in 96 well plates and then MTT is added to cells followed by plate reading at 540 nm in a microplate reader
http://www.pitt.edu/~lazo/MTT.html
Added: Sun Aug 13 2006, Hits: 15688, Reviews: 0 Write review -
Methods for Studying Cell Proliferation and Viability
(Roche)
Provides general background information about methods used for cell proliferation and viability analysis
http://www.roche-applied-science.com/PROD_INF/MANU...
Added: Thu Mar 08 2007, Hits: 7332, Reviews: 0 Write review -
MTT Assay
(Gene Delivery Group, University of Birmingham)
This procedure is for cells in 96 well plates.
http://web.bham.ac.uk/can4psd4/brum/mtt.html
Added: Mon Sep 09 2002, Hits: 11040, Reviews: 2 Read reviews Write review Cached -
MTT assay for cell proliferation
(Shivdasani Lab, Harvard Medical School)
http://research.dfci.harvard.edu/shivdasani/protoc...
Added: Wed Jun 16 2004, Hits: 24816, Reviews: 0 Write review -
MTT Assay Protocol
(Gumbleton Lab, Cardiff University)
Measurement of viable cell numbers using the MTT assay
http://www.cf.ac.uk/phrmy/PCB/LabProtocolsPDFs/Cel...
Added: Fri Jul 20 2007, Hits: 7000, Reviews: 0 Write review -
MTT Cell Proliferation Assay
(ATCC)
The yellow tetrazolium MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) is reduced by metabolically active cells, in part by the action of dehydrogenase enzymes, to generate reducing equivalents such as NADH and NADPH. The resulting intracellular purple formazan can be solubilized and quantified by spectrophotometric means. This protocol, although a manual to a MTT assay kit, can be followed for general use.
http://www.atcc.org/pdf/30-1010k.pdf
Added: Wed Jun 16 2004, Hits: 24462, Reviews: 0 Write review