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3 of 28 people found the following review helpful:
MTT assay on big size cancer cells, Fri Mar 12 2004
Reviewed By: pazhanimuthu
I too fell the same problem with 786-O (renel cell carcinime) It is not responding properly by MTT assay.

May be in this kind of cell line with over spread we can go SRB ( sulphorhodamineB) is betterly working.


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6 of 27 people found the following review helpful:
MTT proliferation, Wed Jun 11 2003
Reviewed By: johnny
i tried this assay with cancer cells (MDAMB231 & PC3), and tried the DMSO to dissolve the formazan, but the cellular debris still remains in the well and i think this causes interference in OD readings. how do you rid of that? or fully lyse the cells along with dissolving the DNA? thanks.

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