| This procedure is for cells in 96 well plates, if larger plates are used then adjust volumes accordingly. 1 Make a solution of 5mg/ml MTT dissolved in PBS and filter sterilise. - 5 hours before the end of the incubation add 20m l of MTT solution from step one to each well containing cells.
- Incubate the plate at 37ºC for 5 hours.
- Remove media with needle and syringe.
- Add 200m l of DMSO to each well and pipette up and down to dissolve crystals.
- Put plate into the 37ºC incubator for 5 minutes to dissolve air bubbles.
Transfer to plate reader and measure absorbance at 550nm. | 